ISOLATION AND CHARACTERIZATION OF SPOROTHRIX-SCHENCKII FROM CLINICAL AND ENVIRONMENTAL SOURCES ASSOCIATED WITH THE LARGEST UNITED-STATES EPIDEMIC OF SPOROTRICHOSIS
ISOLATION AND CHARACTERIZATION OF SPOROTHRIX-SCHENCKII FROM CLINICAL AND ENVIRONMENTAL SOURCES ASSOCIATED WITH THE LARGEST UNITED-STATES EPIDEMIC OF SPOROTRICHOSIS
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DOI：
10.1128/jcm.29.6.1106-1113.1991
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发表时间：
1991-06-01
影响因子：
9.4
通讯作者：
COLES, FB
中科院分区：
文献类型：
--
作者：
DIXON, DM;SALKIN, IF;COLES, FB
美国有记录的最大规模的孢子丝菌病流行发生在 1988 年，涉及 15 个州共 84 例病例。所有病例均与威斯康星州种植的泥炭藓有关。 21 株申克孢子丝菌临床分离株和 69 株孢子丝菌环境分离株。对此次流行病进行了表征和比较。使用直接电镀技术从 102 个泥炭藓和其他材料样品中回收了环境分离物。检查的特征包括宏观和微观形态、向酵母相的转化、外抗原反应和小鼠毒力。根据这些研究，八种环境分离株被鉴定为申克沙门氏菌，五种被鉴定为Ophiostoma stenoceras，其余被鉴定为孢子丝菌种。申克链霉菌的环境分离物是从宾夕法尼亚州一处苗圃和纽约州三个水土保持区的苔藓样本中回收的，但没有直接从威斯康星州沼泽的苔藓中回收到。
The largest recorded epidemic of sporotrichosis in the United States occurred in 1988 and involved a total of 84 cases in 15 states. All cases were associated with Wisconsin-grown sphagnum moss. Twenty-one clinical isolates of Sporothrix schenckii and 69 environmental isolates of Sporothrix spp. from the epidemic were characterized and compared. The environmental isolates were recovered from 102 samples of sphagnum moss and other material by using direct plating techniques. Characteristics examined included macroscopic and microscopic morphology, conversion to a yeast phase, exoantigen reactions, and virulence in mice. On the basis of these studies, eight environmental isolates were identified as S. schenckii, five were identified as Ophiostoma stenoceras, and the remainder were identified as Sporothrix species. The environmental isolates of S. schenckii were recovered from moss samples from one Pennsylvania nursery and from three New York State Soil and Water Conservation districts, but none were recovered from moss directly from the bogs in Wisconsin.