A MUTATED ARO4 GENE FOR FEEDBACK-RESISTANT DAHP SYNTHASE WHICH CAUSES BOTH OMICRON-FLUORO-DL-PHENYLALANINE RESISTANCE AND BETA-PHENETHYL-ALCOHOL OVERPRODUCTION IN SACCHAROMYCES-CEREVISIAE
A MUTATED ARO4 GENE FOR FEEDBACK-RESISTANT DAHP SYNTHASE WHICH CAUSES BOTH OMICRON-FLUORO-DL-PHENYLALANINE RESISTANCE AND BETA-PHENETHYL-ALCOHOL OVERPRODUCTION IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1007/bf00334771
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发表时间：
1991-01-01
期刊：
影响因子：
2.5
通讯作者：
TAKASAWA, S
中科院分区：
文献类型：
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作者：
FUKUDA, K;WATANABE, M;TAKASAWA, S
从酿酒酵母（Saccharomyces cerevisiae）的实验室菌株中分离到过量产生β-苯乙醇的邻氟-DL-苯丙氨酸（OFP）抗性突变体。突变体之一的细胞积累的酪氨酸和苯丙氨酸比野生型细胞多1.5-3倍。其由AR 04编码的3-脱氧-D-阿拉伯-庚糖醛酸-7-磷酸（DAHP）合酶（EC 4.1.2.15）不受酪氨酸的反馈抑制。遗传分析表明，该突变受一个显性基因ARO 4-OFP控制，该基因编码抗反馈酪氨酸DAHP合成酶，该基因同时引起OFP抗性和β-苯乙醇过量生产。这得到了使用来自野生型及其突变株的克隆ARO 4的分子遗传学研究的支持。
o-Fluoro-DL-phenylalanine (OFP)-resistant mutants which overproduce beta-phenethyl-alcohol were isolated from a laboratory strain of Saccharomyces cerevisiae. Cells of one of the mutants accumulated tyrosine and phenylalanine 1.5-3 fold more than did wild-type cells. Its 3-deoxy-D-arabino-hepturosonate-7-phosphate (DAHP) synthase (EC 4.1.2.15), encoded by AR04, was free from feedback inhibition by tyrosine. Genetic analysis revealed that the mutation was controlled by a single dominant gene, ARO4-OFP, encoding feedback-resistant DAHP synthase by tyrosine, and that this gene caused both the OFP resistance and beta-phenethyl-alcohol overproduction. This was supported by molecular genetic studies using cloned ARO4 both from the wild-type and its mutant strain.