Dual in Utero Electroporation in Mice to Manipulate Two Specific Neuronal Populations in the Developing Cortex.
Dual in Utero Electroporation in Mice to Manipulate Two Specific Neuronal Populations in the Developing Cortex.
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DOI：
10.3389/fbioe.2021.814638
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发表时间：
2021
影响因子：
5.7
通讯作者：
Bordey A
中科院分区：
文献类型：
--
作者：
Zhang L;Getz SA;Bordey A
在皮层神经元发育过程中精确调控基因表达对于神经元连接和高阶认知的建立和维持是必不可少的。双子宫内电穿孔提供了一种精确和有效的工具，以空间和时间调节的方式标记和操纵回路内多个神经元群体中的基因表达。此外，这种技术允许形态生理学研究神经元的发育和连接后，细胞特异性基因操作。在这里，我们详细介绍了双子宫内电穿孔协议。
Precise regulation of gene expression during development in cortical neurons is essential for the establishment and maintenance of neuronal connectivity and higher-order cognition. Dual in utero electroporation provides a precise and effective tool to label and manipulate gene expression in multiple neuronal populations within a circuit in a spatially and temporally regulated manner. In addition, this technique allows for morphophysiological investigations into neuronal development and connectivity following cell-specific gene manipulations. Here, we detail the dual in utero electroporation protocol.