Cellular immune responses to human islet proteins in antibody-positive type 2 diabetic patients
Cellular immune responses to human islet proteins in antibody-positive type 2 diabetic patients
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DOI：
10.2337/diabetes.48.5.983
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发表时间：
1999-05-01
期刊：
影响因子：
7.7
通讯作者：
Palmer, JP
中科院分区：
文献类型：
--
作者：
Brooks-Worrell, BM;Juneja, R;Palmer, JP
1型糖尿病是一种细胞介导的自身免疫性疾病，其特征在于自身抗体和外周血单核细胞（PBMC）对胰岛细胞蛋白的反应性。2型糖尿病不是自身免疫性疾病，而是由胰岛素抵抗和非自身免疫性胰岛素分泌缺陷引起的。然而，有一组表型2型糖尿病患者具有与1型糖尿病患者相似的胰岛自身抗体。在这项研究中，我们调查，使用细胞免疫印迹，是否2型糖尿病患者胰岛自身抗体阳性的PBMC对胰岛蛋白的反应。我们观察到，自身抗体阴性（Ab（-））的2型糖尿病患者（n = 9）和正常对照受试者（n = 12）表现出PBMC对0-3个分子量区域的应答，相反，自身抗体阳性（Ab（+））的2型糖尿病患者（n = 11）表现出PBMC对3-18个分子量区域的应答，类似于1型糖尿病患者（响应于4-18个分子量区域）。观察到来自超过90%的Ab（+）2型和1型糖尿病患者的PBMC增殖为97 kDa附近的胰岛蛋白。相反，65-90%的1型糖尿病患者的PBMC在大多数分子量区域中对胰岛蛋白有反应，而
Type 1 diabetes is a cell-mediated autoimmune disease characterized by autoantibody and peripheral blood mononuclear cell (PBMC) reactivity to islet cell proteins. Type 2 diabetes is not an autoimmune disease but rather results from both insulin resistance and a nonautoimmune insulin secretory defect. There is, however, a group of phenotypic type 2 diabetic patients who have islet autoantibodies that are similar to those of type 1 diabetic patients. In this study, we investigated, using cellular immunoblotting, whether type 2 diabetic patients positive for islet autoantibodies have PBMC responses to islet proteins. We observed that autoantibody negative (Ab(-)) type 2 diabetic patients (n = 9) and normal control subjects (n = 12) demonstrated PBMCs responsive to 0-3 molecular weight regions, In contrast, autoantibody positive (Ab(+)) type 2 diabetic patients (n = 11) demonstrated PBMC responses to 3-18 molecular weight regions, similar to that of type 1 diabetic patients (responsive to 4-18 molecular weight regions). PBMCs from over 90% of the Ab(+) type 2 and type 1 diabetic patients were observed to proliferate to islet proteins in the vicinity of 97 kDa, In contrast, 65-90% of type 1 diabetic patients had responsive PBMCs for islet proteins in most of the molecular weight regions, whereas