SEQUENCE-SPECIFIC ISOTOPE EFFECTS ON THE CLEAVAGE OF DNA BY BLEOMYCIN
SEQUENCE-SPECIFIC ISOTOPE EFFECTS ON THE CLEAVAGE OF DNA BY BLEOMYCIN
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DOI：
10.1126/science.2476851
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发表时间：
1989-09-22
期刊：
影响因子：
56.9
通讯作者：
STUBBE, J
中科院分区：
文献类型：
--
作者：
KOZARICH, JW;WORTH, L;STUBBE, J
博莱霉素是一种依赖金属和氧气的DNA切割器。DNA损伤的化学被认为涉及4‘-氢的限速提取。制备了一段含有高同位素含量的[4‘’-2H]胸苷残基的DNA片段。用DNA测序技术直接在单个胸苷残基上观察到了主要的动力学同位素效应。
Bleomycin is a metal- and oxygen-dependent DNA cleaver. The chemistry of DNA damage has been proposed to involve rate-limiting abstraction of the 4''-hydrogen. A DNA fragment has been prepared that contains [4''-2H]thymidine residues of high isotopic content. Primary kinetic isotope effects have been directly observed at individual thymidine residues with DNA sequencing technology.