Characterization of the bacterial communities associated with the bald sea urchin disease of the echinoid Paracentrotus lividus
Characterization of the bacterial communities associated with the bald sea urchin disease of the echinoid Paracentrotus lividus
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DOI：
10.1016/j.jip.2007.12.002
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发表时间：
2008-06-01
影响因子：
3.4
通讯作者：
Eeckhaut, Igor
中科院分区：
文献类型：
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作者：
Becker, Pierre T.;Egea, Emilie;Eeckhaut, Igor
利用非培养技术研究了海胆Paracentrotus lividus秃海胆病的微生物群落。采用扫描电子显微镜（SEM）对法国La Ciotat（地中海）和Morgat（大西洋）两个地点的病变标本进行了检查，并通过变性梯度凝胶电泳（DGGE）和16 S rRNA基因克隆文库构建分析了其微生物群的多样性。显微镜观察表明，只有病变的中心区域被细菌侵入，而周围区域和周围健康组织则没有。分子分析确定了至少24个细菌基因型在秃海胆病变：5个是Alphaproteohacteria，10个是Gammaproteobacteria，8个是CFB细菌和我是梭菌。其中，4个在两个地点都有观测到，10个只出现在大西洋，10个只出现在地中海。γ-变形菌在两个地点的克隆文库中最具代表性，分别占总克隆的65%和43%。CFB和Alphaproteobacteria占大多数的其余克隆，并检测DGGE几乎所有的样品从两个站。我们的研究结果表明，细菌群落观察到的患病个体的相同的海胆物种，但来自不同的位置是不相似的，从而支持的假设，即细菌参与在世界范围内的海胆疾病通常被称为秃头海胆病是机会性的，而不是具体的。(c)2007爱思唯尔公司All rights reserved.
The microbial communities involved in the bald sea urchin disease of the echinoid Paracentrotus lividus are investigated using culture-independent techniques. Lesions of diseased specimens from two locations in France, La Ciotat (Mediterranean Sea) and Morgat (Atlantic Ocean), are examined by Scanning Electron Microscopy (SEM) and the diversity of their microbiota is analysed by Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rRNA gene clones libraries construction. Microscopic observations demonstrated that only the central area of the lesions is invaded by bacteria but not the peripheral zone and the surrounding healthy tissues. Molecular analysis identified at least 24 bacterial genomospecies in bald sea urchin lesions: 5 are Alphaproteohacteria, 10 are Gammaproteobacteria, 8 are CFB bacteria and I is a Fusobacteria. Out of them, 4 are observed in both locations while 10 occur only in the Atlantic Ocean and 10 only in the Mediterranean Sea. Gammaproteobacteria are the most represented in clones libraries from both locations, with respectively 65% and 43% of the total clones. CFB and Alphaproteobacteria accounted for the majority of the remaining clones and were detected by DGGE in virtually all samples from both stations. Our results demonstrate that bacterial communities observed on diseased individuals of the same echinoid species but originating from distinct locations are not similar and thus support the hypothesis that bacteria involved in the worldwide echinoid disease commonly called the bald sea urchin disease are opportunistic and not specific. (c) 2007 Elsevier Inc. All rights reserved.