Light damage as a model of retinal degeneration.
Light damage as a model of retinal degeneration.
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DOI：
10.1007/978-1-62703-080-9_6
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Reme, Charlotte E
中科院分区：
文献类型：
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作者：
Grimm, Christian;Reme, Charlotte E
通过光暴露诱导视网膜变性被广泛用于研究细胞死亡的机制。这种光诱导的损伤相对于遗传决定的退化的优势在于，可以根据实验者的需要来操纵光暴露。明亮的白色光暴露可以在大的视网膜区域中诱导光感受器中细胞凋亡的同步爆发，这允许以受控的方式研究细胞和分子事件。高能量的蓝光在非常短的曝光持续时间（秒到分钟）内诱导高视网膜辐照度的热点，并且可以帮助解开光吸收后的初始事件，这对于所有损伤方案来说可能是相似的。然后，这些初始事件可以诱导各种分子信号传导途径和次级效应，例如脂质和蛋白质氧化，其可以分别在不同的光损伤设置和不同的菌株或物种中变化。蓝光损伤还允许研究与周围组织相比，在限定的视网膜区域（热点）中的细胞反应。在这里，我们描述了短时间暴露于明亮的全光谱白色光（在数小时范围内）和短时间暴露于高能单色蓝色或绿色光（数秒至数分钟）的方法。
The induction of retinal degeneration by light exposure is widely used to study mechanisms of cell death. The advantage of such light-induced lesions over genetically determined degenerations is that light exposures can be manipulated according to the needs of the experimenter. Bright white light exposure can induce a synchronized burst of apoptosis in photoreceptors in a large retinal area which permits to study cellular and molecular events in a controlled fashion. Blue light of high energy induces a hot spot of high retinal irradiance within very short exposure durations (seconds to minutes) and may help to unravel the initial events after light absorption which may be similar for all damage regimens. These initial events may then induce various molecular signaling pathways and secondary effects such as lipid and protein oxidation, which may be varying in different light damage setups and different strains or species, respectively. Blue light lesions also allow to study cellular responses in a circumscribed retinal area (hot spot) in comparison with the surrounding tissue.Here we describe the methods for short-term exposures (within the hours range) to bright full-spectrum white light and for short exposures (seconds to minutes) to high-energy monochromatic blue or green light.