Dephosphorylation of LjMPK6 by Phosphatase LjPP2C is Involved in Regulating Nodule Organogenesis inLotus japonicus
Dephosphorylation of LjMPK6 by Phosphatase LjPP2C is Involved in Regulating Nodule Organogenesis inLotus japonicus
复制标题
磷酸酶 LjPP2C 对 LjMPK6 的去磷酸化参与调节莲花根瘤器官发生
DOI：
10.3390/ijms21155565
复制
发表时间：
2020-08-01
影响因子：
5.6
通讯作者：
Duanmu, Deqiang
中科院分区：
文献类型：
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作者：
Yan, Zhongyuan;Cao, Jingjing;Duanmu, Deqiang
丝裂原活化蛋白激酶(MAPK)LjMPK6是SIP2的磷酸化靶标，SIP2是一种MAPK激酶，与SymRK(共生受体样激酶)相互作用，调节豆类根瘤菌的共生。LjMPK6和SIP2都是莲花结瘤所必需的。然而，LjMPK6及其调控成分在根瘤发育中的去磷酸化仍未被探索。通过酵母双杂交筛选，我们在体外鉴定出一种能与LjMPK6特异性相互作用并使其去磷酸化的2C型蛋白磷酸酶LjPP2C。生理生化分析进一步表明，LjPP2C磷酸酶是体内LjMPK6去磷酸化和根瘤菌接种后微调根瘤发育所必需的。一个不可磷酸化的突变体LjMPK6(T224A Y226F)可以模拟LjPP2C在毛状根转化植株根瘤发育所需的MAPK去磷酸化中的功能。总之，我们的研究表明，LjMPK6的去磷酸化需要与LjPP2C磷酸酶相互作用，以微调INL的根瘤发育。日本鱼。
The mitogen-activated protein kinase (MAPK) LjMPK6 is a phosphorylation target of SIP2, a MAPK kinase that interacts with SymRK (symbiosis receptor-like kinase) for regulation of legume-rhizobia symbiosis. Both LjMPK6 and SIP2 are required for nodulation inLotus japonicus. However, the dephosphorylation of LjMPK6 and its regulatory components in nodule development remains unexplored. By yeast two-hybrid screening, we identified a type 2C protein phosphatase, LjPP2C, that specifically interacts with and dephosphorylates LjMPK6 in vitro. Physiological and biochemical assays further suggested that LjPP2C phosphatase is required for dephosphorylation of LjMPK6 in vivo and for fine-tuning nodule development after rhizobial inoculation. A non-phosphorylatable mutant variant LjMPK6 (T224A Y226F) could mimic LjPP2C functioning in MAPK dephosphorylation required for nodule development in hairy root transformed plants. Collectively, our study demonstrates that interaction with LjPP2C phosphatase is required for dephosphorylation of LjMPK6 to fine tune nodule development inL. japonicus.