Wood creosote prevents CRF-induced motility via 5-HT3 receptors in proximal and 5-HT4 receptors in distal colon in rats
Wood creosote prevents CRF-induced motility via 5-HT3 receptors in proximal and 5-HT4 receptors in distal colon in rats
复制标题
DOI：
10.1016/j.autneu.2006.11.002
复制
发表时间：
2007-05-30
影响因子：
2.7
通讯作者：
Fujimiya, Mineko
中科院分区：
文献类型：
--
作者：
Ataka, Koji;Kuge, Tomoo;Fujimiya, Mineko
木杂酚油已被用作治疗食物中毒引起的急性腹泻的草药，并对结肠运动和肠毒素诱导的离子分泌具有抑制作用。由于没有以前的研究木杂酚油对结肠运动的应激诱导的改变的影响，我们研究了结肠运动的影响改变脑室内（i.c.v）注射促肾上腺皮质激素释放因子（CRF），这是一个关键的调解人在应激反应。我们通过两个测压导管记录了无限制清醒大鼠近端和远端结肠的运动活动。测量近端和远端结肠的类III相收缩频率和%运动指数。同时，对排泄的粪粒数量进行计数，静脉注射CRF可增加近端和远端结肠的运动活动，这些作用可被静脉注射选择性CRF I型拮抗剂完全拮抗，但不能被CRF 2型拮抗剂拮抗。CRF引起的结肠动力改变可被静脉注射木酚油逆转。肠腔内给予5-HT 3受体拮抗剂格拉司琼或5-HT 4受体拮抗剂SB 204070可阻断静脉注射CRF引起的结肠运动增加。木杂酚油阻止了近端结肠中由5-HT 3受体激动剂SR 57227 A诱导的结肠运动性增加，同时阻止了远端结肠中由5-HT 4受体激动剂RS67506诱导的结肠运动性增加。这些结果表明，木杂酚油通过近端结肠中的5-HT 3受体和远端结肠中的5-HT 4受体阻止由CRF诱导的结肠运动的增加，这表明木杂酚油可能对治疗应激诱导的腹泻有用。(C)2006 Elsevier B. V.保留所有权利。
Wood creosote has been used as an herbal medicine against acute diarrhea caused by food poisoning and has an inhibitory effect on colonic motility and enterotoxin-induced ion secretion. Since no previous studies have examined the effects of wood creosote on stress-induced alteration of colonic motility, we examined the effects on the colonic motility altered by intracerebroventricular (i.c.v) injection of corticotropin-releasing factor (CRF), which is a key mediator in responses to stress. We recorded motor activity in proximal and distal colon of unrestrained conscious rats via two manometory catheters. The frequencies of phase III-like contraction and the % motor indices in both proximal and distal colon were measured. At the same time the number of fecal pellets excreted was counted, I.c.v. injection of CRF increased the motor activity in both proximal and distal colon, and these effects were completely antagonized by i.c.v. injection of a selective CRF type I antagonist but not by a CRF type 2 antagonist. Changes in colonic motility induced by CRF were reversed by intravenously administered wood creosote. Intraluminal administration of the 5-HT3 receptor antagonist granisetron, or the 5-HT4 receptor antagonist SB 204070 blocked the increase in colonic motility induced by i.c.v. injection of CRF. Wood creosote prevented the increase in colonic motility induced by the 5-HT3 receptor agonist SR57227A in the proximal colon, while it prevented the increase in colonic motility induced by the 5-HT4 receptor agonist RS67506 in the distal colon. These results indicate that wood creosote prevents the increase in colonic motility induced by CRF via 5-HT3 receptors in the proximal colon, and via 5-HT4 receptors in the distal colon, suggesting that wood creosote might be useful to treat stress-induced diarrhea. (C) 2006 Elsevier B.V. All rights reserved.