In Vitro Cytotoxic Effect of Ethanol Extract Prepared From Sporophyll of Undaria pinnatifida on Human Colorectal Cancer Cells
In Vitro Cytotoxic Effect of Ethanol Extract Prepared From Sporophyll of Undaria pinnatifida on Human Colorectal Cancer Cells
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裙带菜孢子叶乙醇提取物对人结直肠癌细胞的体外细胞毒作用
DOI：
10.1002/ptr.3527
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发表时间：
2012
影响因子：
7.2
通讯作者：
Naoyoshi Nishibori
中科院分区：
文献类型：
--
作者：
Akamatsu;R;外山未來，安部景奈，赤松利恵;Gaojian Lian;Naoyoshi Nishibori
裙带菜（Undaria pinnatifida（Harvey）Suringar）是一种广受欢迎的食品，在东亚国家用于医疗保健。这种海藻的主要成分被证明有益于高血压和高脂血症，并被认为可以降低梗塞和缺血性疾病的风险。此外，海藻膳食纤维的摄入被认为可以防止胃肠道癌症的产生和扩散。研究了从裙带菜孢子叶（mekabu）制备的乙醇提取物对HCT 116人结直肠癌细胞的直接作用，并且显示mekabu提取物诱导细胞的非氧化性凋亡损伤，从而以浓度依赖性方式导致其活力降低。此外，仅在含血清的培养基中观察到抑癌药物（如5-氟尿嘧啶（5-FU）和伊立替康（CPT-11））的细胞毒性作用，而mekabu提取物即使在无血清培养基中也能有效降低细胞活力。这些发现表明，mekabu提取物可能含有潜在的活性物质，可能通过与5-FU和CPT-11不同的机制诱导非氧化性凋亡细胞死亡，因此mekabu可能用作结直肠癌化疗的辅助药物。版权所有© 2011约翰威利父子有限公司.
Brown seaweedUndaria pinnatifida(Harvey) Suringar is popular as a foodstuff, and used for medical care in East Asian countries. The major components of this seaweed are shown to benefit hypertension and hyperlipidemia, and considered to reduce the risks of infarction and ischemic diseases. Furthermore, the intake of dietary fiber of seaweeds is considered to prevent the production and proliferation of cancer in the gastrointestinal tract. The direct effect of an ethanol extract prepared fromUndaria pinnatifidasporophyll (mekabu) on HCT116 human colorectal cancer cells was examined, and the mekabu extract was shown to induce the non‐oxidative apoptotic damage to the cells, thus resulting in the reduction of their viabilities in a concentration‐dependent manner. Moreover, the cytotoxic effects of carcinostatic drugs, such as 5‐fluorouracil (5‐FU) and irinotecan (CPT‐11), were observed only in the medium containing sera, while the mekabu extract could effectively reduce the cell viabilities even in the serum‐free medium. These findings suggest that the mekabu extract may contain a potential active substance inducing the non‐oxidative apoptotic cell death probably through a mechanism different from those of 5‐FU and CPT‐11, and hence mekabu is possibly useful as an auxiliary drug to the chemotherapy of colorectal cancer. Copyright © 2011 John Wiley & Sons, Ltd.