Mouse sperm antigens that participate in fertilization. III. Passive immunization with a single monoclonal antisperm antibody inhibits pregnancy and fertilization in vivo.
Mouse sperm antigens that participate in fertilization. III. Passive immunization with a single monoclonal antisperm antibody inhibits pregnancy and fertilization in vivo.
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参与受精的小鼠精子抗原。
DOI：
10.1095/biolreprod33.2.537
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发表时间：
1985
影响因子：
3.6
通讯作者：
Waibel,R
中科院分区：
文献类型：
--
作者：
Saling,PM;Waibel,R
采用被动免疫方法研究抗小鼠精子单克隆抗体对体内受精的影响。本研究比较了两种抗体的作用。其中之一，M29，先前已被证明定位于精子头的赤道部分，并以浓度依赖的方式抑制小鼠体外受精。第二种抗体M2与精子头部的相同区域结合，也属于M免疫球蛋白类（IgM），但不影响体外受精。在妊娠母马血清促性腺激素和人绒毛膜促性腺激素注射的同时，腹腔注射两次浓度为0.5-4.0 mg IgM或对照IgG的抗体；动物在注射hCG后6-12小时内交配。仅在接种了M29 IgM的动物中（例如，4 mg M29 IgM: 304个受精卵中的12.6%；4 mg M2 IgM: 192个受精卵中的96%），受精卵和伴随妊娠的建立以剂量依赖的方式显著减少。腹腔注射抗体不会降低超排卵水平和卵母细胞活力。125i标记的M29 IgM用于测定被动免疫小鼠受精卵时输卵管壶腹内抗体的含量。腹腔内M29 IgM与腹腔内剂量呈线性关系：在hcg后14-16小时，输卵管内存在注射剂量的0.002-0.003%。将M29在体内条件下的抑制效果与体外条件下的抑制效果进行比较（Siding, Irons, and Waibel，本问题），发现两种实验体系具有高度的一致性。这些结果是使用识别单个精子表位的单克隆抗体获得的，这为开发使用参与受精过程的特定精子抗原的避孕疫苗提供了可能的基础。
Passive immunization was used to study the effect of antimouse sperm monoclonal antibodies on fertilization in vivo. The effects of two antibodies were compared in this investigation. One of them, M29, has been shown previously to localize to the equatorial segment of the sperm head and to inhibit mouse fertilization in vitro in a concentration-dependent manner. The second antibody, M2, binds to the same area of the sperm head, and also belongs to the M immunoglobulin class (IgM), but does not affect fertilization in vitro. Superovulated female mice received two antibody injections intraperitoneally (at the times of the pregnant mare’s serum gonadotropin and human chorionic gonadotropin injections) at concentrations of 0.5–4.0 mg of IgM or control IgG; animals were mated within 6–12 h of the hCG injection. Fertilization and concomitant establishment of pregnancy were reduced significantly, in a dose-dependent manner, only in those animals immunized with M29 IgM (e.g., 4 mg M29 IgM: 12.6% of 304 eggs fertilized; 4 mg M2 IgM: 96% of 192 eggs fertilized). Intraperitoneal administration of the antibodies did not depress superovulation levels nor oocyte viability.125I-labeled M29 IgM was used to determine the amount of antibody present in the oviductal ampulla at the time of fertilization in passively immunized mice. Luminal M29 IgM was found to be a linear function of the intraperitoneal dose: 0.002–0.003% of the injected dose was present in the oviductal lumen 14–16 h post-hCG. When the inhibitory efficacy of M29 under in vivo conditions was compared to that under in vitro conditions (Siding, Irons, and Waibel, this issue), a high degree of correspondence was found for the two experimental systems. These results, achieved using a monoclonal antibody that recognizes a single sperm epitope, suggest a possible basis for the development of a contraceptive vaccine employing specific sperm antigens that participate in the fertilization process.