In Vitro Models of the Blood-Brain Barrier.
In Vitro Models of the Blood-Brain Barrier.
复制标题
血脑屏障的体外模型。
DOI：
10.1007/978-1-0716-2289-6_2
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Cucullo,Luca
中科院分区：
文献类型：
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作者：
Raut,Snehal;Bhalerao,Aditya;Noorani,Behnam;Cucullo,Luca
传统的体外模型可以复制药物跨血脑屏障（BBB）转运/渗透的许多基本特征，但不能完全预测体内中枢神经系统（CNS）的摄取。物种差异未能将实验疗法从研究实验室转化为临床。改善人血脑屏障的体外模型对于中枢神经系统药物的发现和递送都是至关重要的。通过微流体技术制造的高端人类血脑屏障模型为这个问题提供了一些解决方案。BBB的复杂生理微环境已经通过增加多细胞、动态条件和3D设计方面的设备复杂性而建立。现在可以预测候选药物的治疗效果，并通过使用先进的体外BBB模型研究多细胞相互作用来确定新的药物靶点。本章回顾了目前以及一个理想的体外模型的血脑屏障。
Traditional in vitro models can replicate many essential features of drug transport/permeability across the blood-brain barrier (BBB) but are not entirely projecting in vivo central nervous system (CNS) uptake. Species differences fail to translate experimental therapeutics from the research laboratory to the clinic. Improved in vitro modeling of human BBB is vital for both CNS drug discovery and delivery. High-end human BBB models fabricated by microfluidic technologies offer some solutions to this problem. BBB’s complex physiological microenvironment has been established by increasing device complexity in terms of multiple cells, dynamic conditions, and 3D designs. It is now possible to predict the therapeutic effects of a candidate drug and identify new druggable targets by studying multicellular interactions using the advanced in vitro BBB models. This chapter reviews the current as well as an ideal in vitro model of the BBB.