Spatiotemporal expression of UPK3B and its promoter activity during embryogenesis and spermatogenesis
Spatiotemporal expression of UPK3B and its promoter activity during embryogenesis and spermatogenesis
复制标题
DOI：
10.1007/s00418-016-1486-8
复制
发表时间：
2017-01-01
影响因子：
2.3
通讯作者：
Tanaka, Masamitsu
中科院分区：
文献类型：
--
作者：
Kuriyama, Sei;Tamiya, Yuutaro;Tanaka, Masamitsu
尿血小板溶素（Upk）3是尿路上皮组织的主要结构成分之一。尽管与UPK3A相比，UPK3B在更多种类的组织和器官中均有表达，但其组织特异性表达尚未得到分析。在此，我们分析了转基因小鼠中由Upk3b启动子驱动的Cre重组酶活性以及UPK3B的内源性定位。我们通过将ROSA26（tm14（CAG - tdTomato））（R26（tdTomato））小鼠与Tg（Upk3b - Cre）小鼠杂交，培育出了Tg（Upk3b - Cre）/R26（tdTomato）小鼠，并研究了tdTomato在胚胎期和成年小鼠中的时空分布。在胚胎中，我们在神经嵴细胞以及心脏、肝脏、肾脏和肺中检测到了Cre重组酶活性。在成年小鼠中，在雄性和雌性生殖器官中检测到了Cre重组酶活性；然而，在膀胱中未检测到该活性。组织学分析显示，tdTomato和UPK3B在睾丸和附睾精子中均有存在；然而，在检测到UPK3B内源性表达的附睾管中未发现tdTomato。在雌性小鼠中，tdTomato和UPK3B的表达均在卵巢卵泡中被检测到，而在检测到内源性UPK3B表达的输卵管黏膜上皮中未发现tdTomato表达。这些数据表明，UPK3B可能在配子及配子输送器官的成熟过程中发挥关键作用。
Uroplakin (Upk) 3 is one of the main structural components of the urothelium tissue. Although expression of UPK3B is seen in a wider variety of the tissues and organs than UPK3A, tissue-specific expression has not yet been analyzed. Here, we analyzed the Cre recombinase activity driven by the Upk3b promoter in transgenic mice and the endogenous localization of UPK3B. We generated Tg(Upk3b-Cre)/R26(tdTomato) mice by crossing ROSA26(tm14(CAG-tdTomato)) (R26(tdTomato)) mice with Tg(Upk3b-Cre) mice and investigated the spatiotemporal distribution of tdTomato in embryonic and adult mice. In embryos, we detected Cre recombinase activity in neural crest cells and the heart, liver, kidneys, and lungs. In adult mice, Cre recombinase activity was detected in male and female genital organs; however, the activity was absent in the bladder. Histological analyses revealed that both tdTomato and UPK3B were present in testicular and epididymal sperm; however, tdTomato was not present in the ductus epididymis, where the endogenous expression of UPK3B was detected. In female siblings, both tdTomato and UPK3B expressions were detected in the follicles of the ovary, whereas no tdTomato expression was found in the mucosal epithelium of the fallopian tubes, where the endogenous UPK3B was expressed. These data suggest that UPK3B may play a pivotal role in the maturation of gametes and gamete-delivery organs.