TRANSFORMING GROWTH-FACTOR-BETA REGULATES THE SPLICING PATTERN OF FIBRONECTIN MESSENGER-RNA PRECURSOR
TRANSFORMING GROWTH-FACTOR-BETA REGULATES THE SPLICING PATTERN OF FIBRONECTIN MESSENGER-RNA PRECURSOR
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DOI：
10.1016/0014-5793(90)80664-5
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发表时间：
1990-02-12
期刊：
影响因子：
3.5
通讯作者：
ZARDI, L
中科院分区：
文献类型：
--
作者：
BORSI, L;CASTELLANI, P;ZARDI, L
纤连蛋白（FN）多态性是由单个基因的初级转录本的至少三个区域（ED-A、ED-B和IIICS）中的选择性剪接模式引起的。使用单克隆抗体，我们先前证明转化生长因子-β（TGF-β）优先增加含有ED-A序列的FN同种型在培养的正常人成纤维细胞中的积累[Balza等人，04 The Dog（1988）228，42-44]。为了确定这种效应的基础，我们通过S1核酸酶分析检测了TGF-β处理前后培养的正常人皮肤成纤维细胞中含ED-A和ED-B的mRNA的水平。这些实验表明，TGF-β增加了含有ED-A和ED-B的FN同种型的m-RNA的相对量。这些数据表明，生长因子可以调节前mRNA的剪接模式。
Fibronectin (FN) polymorphism is caused by alternative splicing patterns in at least three regions (ED-A, ED-B and IIICS) of the primary transcript of a single gene. Using monoclonal antibodies, we previously demonstrated that transforming growth factor-β (TGF-β) preferentially increases the accumulation of the FN isoforms containing the ED-A sequence in cultured normal human fibroblasts [Balza et al., (1988) FEBS Lett. 228, 42-44]. To determine the basis of this effect, we have examined through S1 nuclease analysis, the levels of ED-A- and ED-B-containing mRNAs in cultured normal human skin flbroblasts before and after TGF-β treatment. These experiments have shown that TGF-β increases the relative amount of m-RNA for ED-A- and ED-B-containing FN isoforms. These data demonstrate that a growth factor may regulate the splicing pattern of a pre-mRNA.