Molecular cloning and sequence characterization of two genes, DQA1 and DQA2, from the Chinese yakow (Bos grunniens x Bos taurus)
Molecular cloning and sequence characterization of two genes, DQA1 and DQA2, from the Chinese yakow (Bos grunniens x Bos taurus)
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中国牦牛（Bos grunniens x Bos taurus）两个基因 DQA1 和 DQA2 的分子克隆和序列表征
DOI：
10.3906/vet-1510-86
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发表时间：
2017-02
影响因子：
0.6
通讯作者：
Deng WD
中科院分区：
文献类型：
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作者：
Xi Dongmei;Memon Sameeullah;Li Guozhi;Liu Xiangying;Su Chao;Leng Jing;Wang Liping;Deng Weidong;Xi Dongmei;Memon Sameeullah;Li Guozhi;Liu Xiangying;Su Chao;Leng Jing;Wang Liping;Deng Weidong;Khan Sehroon;Khan Sehroon;Deng WD;Deng WD
主要组织相容性复合体（MHC）在感染性疾病的免疫应答中起着至关重要的作用。本研究的目的是探讨中国牦牛MHC-DQA基因的结构和功能特点，这是很好地适应青藏高原和可能的重复。检索到两个全长cDNA序列和部分5 '和3'非翻译区（UTR），并分别表示为BogrxBoLA-DQA星星0101（DQA 1）和BogrxBoLA-DQA星星2001（DQA 2）。BogrxBoLA-DQA 1和BogrxBoLA-DQA 2之间的核苷酸和氨基酸序列同源性百分比表明，这两个序列与来自其他对应物的相应DQA 1和DQA 2基因的等位基因的同源性高于彼此。此外，BogrxBoLA-DQA 1和-DQA 2表现出相当大的变异，沿着编码区长度具有沿着99个核苷酸多态性，导致52个氨基酸多态性。系统发育树分析表明，这两个基因之间的遗传距离大于不同动物的同源基因之间的遗传距离，表明这两个序列在中国牦牛中属于非等位和重复类型。这是首次分离鉴定MHC-DQA 1和MHC-DQA 2基因。这一结果将加深我们对基于比较群体遗传学的不同反刍动物物种间MHC多样性的理解。
The major histocompatibility complex (MHC) plays a crucial role in the immune response to infectious diseases. The aim of the present study was to investigate the structural and functional characteristics and possible duplication of the MHC-DQA genes in the Chinese yakow, which is well adapted to the Qinghai-Tibetan Plateau. Two full-length cDNA sequences and partial 5'-and 3'-untranslated regions (UTRs) were retrieved and denoted as BogrxBoLA-DQA star 0101 (DQA1) and BogrxBoLA-DQA star 2001 (DQA2), respectively. The percentage of nucleotide and amino acid sequences homology between BogrxBoLA-DQA1 and BogrxBoLA-DQA2 showed that these two sequences have more identity to alleles of the corresponding DQA1 and DQA2 genes from other counterparts than to each other. Moreover, the BogrxBoLA-DQA1 and -DQA2 exhibited considerable variation with 99 nucleotide polymorphisms along the length of the coding regions, leading to 52 amino acid polymorphisms. The phylogenetic tree investigation also demonstrated that there is a larger genetic gap within these two identified genes than within orthologous genes from different animals, suggesting that these sequences belong to nonallelic and duplicated types in the Chinese yakow. This is the first report to isolate and identify the MHC-DQA1 and-DQA2 genes. This result will deepen our understanding of MHC diversity among different ruminant species based on comparative population genetics.