A MICROCALORIMETRIC METHOD TO STUDY THE EFFECT OF KANAMYCIN ON THE METABOLISM OF RECOMBINANT ESCHERICHIA COLI
A MICROCALORIMETRIC METHOD TO STUDY THE EFFECT OF KANAMYCIN ON THE METABOLISM OF RECOMBINANT ESCHERICHIA COLI
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DOI：
10.1080/00986440701242832
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发表时间：
2007-06
影响因子：
2.5
通讯作者：
D. Fan;Li H. Wang;L. Shang;Huidong Shi;X. Ma;Y. Mi;Li-Feng Gu;K. Xu
中科院分区：
文献类型：
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作者：
D. Fan;Li H. Wang;L. Shang;Huidong Shi;X. Ma;Y. Mi;Li-Feng Gu;K. Xu
利用微量热技术研究了卡那霉素对重组大肠杆菌生长和代谢的影响。用不同剂量的卡那霉素处理生长中的重组大肠杆菌细胞悬液，记录其功率-时间曲线，并用广义Logistic方程进行了非常准确的描述。速率常数（k）是卡那霉素浓度依赖性的，并且k与C（卡那霉素剂量）之间的关系接近线性。50%抑制浓度IC 50为88.49 µg/mL。实验结果表明，高剂量的卡那霉素对重组大肠杆菌的生长有抑制作用。大肠杆菌在对数生长期和促进生长在稳定期。这些结果对于确定最大合成类人胶原的最佳卡那霉素剂量是重要的。
A microcalorimetric technique based on the metabolic heat production from cultured cells was used to investigate the effect of kanamycin on the growth and metabolism of recombinant Escherichia coli. Power-time curves of growing recombinant Escherichia coli cell suspensions treated with different kanamycin doses were recorded and were described very accurately by the generalized logistic equation. The rate constant (k) is kanamycin concentration-dependent and the relationship between k and C (kanamycin dose) is nearly linear. The 50% inhibitory concentration IC50 was 88.49 µg/mL. The experimental results revealed that high doses of kanamycin inhibited the growth of recombinant E. coli during the log phase and promoted growth during the stationary phase. These results are important for deciding the optimum kanamycin dose for the maximal synthesis of human-like collagen.