Determination of macromolecular concentration following direct infusion into hydrogel using contrast-enhanced MRI.
Determination of macromolecular concentration following direct infusion into hydrogel using contrast-enhanced MRI.
复制标题
使用对比增强 MRI 直接注入水凝胶后测定大分子浓度。
DOI：
10.1109/iembs.2007.4352932
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Sarntinoranont,Malisa
中科院分区：
文献类型：
--
作者：
Chen,Xiaoming;Astary,GarrettW;Mareci,ThomasH;Sarntinoranont,Malisa
直接组织输注已经成为治疗神经系统疾病的有前途的药物递送方法，因为血脑或血脊髓屏障被绕过。确定输注后治疗药物的空间分布对于评价治疗效果和优化输注方案具有重要意义。在这项研究中，我们提供了一种方法来确定Gd标记的示踪剂的浓度分布，使用对比增强MRI。使用11.1T磁体系统对Gd-DTPA标记的白蛋白（Gd-白蛋白）输注到琼脂糖基水凝胶中进行成像。通过使用来自初步扫描的数据，从MR图像的信号强度确定Gd-白蛋白分布。作为初始验证试验，将这些浓度曲线与对流和扩散多孔介质运输预测的分布曲线进行了比较。模型结果的比较显示预测分布之间具有良好的相关性。在未来的研究中，所提出的方法可用于估计Gd示踪剂的分布后，直接注入组织。
Direct tissue infusion has emerged as a promising drug delivery method for treating diseases of the nervous system because the blood-brain or blood-spinal cord barriers are circumvented. Determination of the spatial distribution of therapeutic agents after infusion is important in evaluating the efficacy of treatment and optimizing infusion protocols. In this study, we provide a methodology to determine the concentration distribution of Gd-labeled tracers using contrast- enhanced MRI. An 11.1 T magnet system was used to image infusion of Gd-DTPA labeled albumin (Gd-albumin) into an agarose-based hydrogel. By using data from preliminary scans, Gd-albumin distribution was determined from the signal intensity of the MR images. As an initial validation test, these concentration profiles were compared with distribution profiles predicted for porous media transport by convection and diffusion. Comparison of model results show good correlation between predicted distributions. In future studies, the presented methodology may be used to estimate the distribution of Gd-tracer following infusion directly into tissue.