Characterization of Pax3-expressing cells from adult blood vessels
Characterization of Pax3-expressing cells from adult blood vessels
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DOI：
10.1242/jcs.085373
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发表时间：
2011-12-01
影响因子：
4
通讯作者：
Buckingham, Margaret
中科院分区：
文献类型：
--
作者：
Goupille, Olivier;Pallafacchina, Giorgia;Buckingham, Margaret
我们报告表达的Pax 3，骨骼肌干细胞的行为的重要调节，在成年小鼠的肱动脉和股动脉。在Pax 3（GFP/+）小鼠中，在肢体的这些收缩动脉中，但在躯干的弹性动脉中没有观察到GFP阳性细胞带。血管的组织学和生物化学检查，以及通过流式细胞术纯化Pax 3-GFP阳性细胞后的克隆分析，建立了它们的血管平滑肌身份。这些血管来源的细胞对其他中胚层细胞类型（如骨）的诱导剂没有反应，然而，当与骨骼肌细胞共培养时，它们可以促进肌纤维形成。这种肌源性转化依赖于Pax 3的表达，但由于需要细胞融合，因此是罕见的和非细胞自主的。Myocardin，促进收购的成熟平滑肌表型在这些Pax 3-GFP阳性细胞，拮抗其潜力骨骼肌分化。然而，基因操作显示，与其他心肌蛋白相关因子MRTFA、MRTFB或SRF的基因不同，心肌蛋白受Pax 3正调控。Pax 3的表达与Msx 2的报道重叠，Msx 2是血管来源的多能中血管母细胞的平滑肌分化所需的。这些观察结果进行了讨论相对于Pax 3表达细胞在血管中的起源和功能，以及更一般的问题，细胞命运的决定和成人细胞的可塑性和重编程。
We report expression of Pax3, an important regulator of skeletal muscle stem cell behaviour, in the brachial and femoral arteries of adult mice. In these contractile arteries of the limb, but not in the elastic arteries of the trunk, bands of GFP-positive cells were observed in Pax3(GFP/+) mice. Histological and biochemical examination of the vessels, together with clonal analysis after purification of Pax3-GFP-positive cells by flow cytometry, established their vascular smooth muscle identity. These blood-vessel-derived cells do not respond to inducers of other mesodermal cell types, such as bone, however, they can contribute to muscle fibre formation when co-cultured with skeletal muscle cells. This myogenic conversion depends on the expression of Pax3, but is rare and non-cell autonomous as it requires cell fusion. Myocardin, which promotes acquisition of a mature smooth muscle phenotype in these Pax3-GFP-positive cells, antagonises their potential for skeletal muscle differentiation. Genetic manipulation shows that myocardin is, however, positively regulated by Pax3, unlike genes for other myocardin-related factors, MRTFA, MRTFB or SRF. Expression of Pax3 overlaps with that reported for Msx2, which is required for smooth muscle differentiation of blood vessel-derived multipotent mesoangioblasts. These observations are discussed with respect to the origin and function of Pax3-expressing cells in blood vessels, and more general questions of cell fate determination and adult cell plasticity and reprogramming.