Major capsid protein of Autographa californica multiple nucleopolyhedrovirus contributes to the promoter activity of the very late viral genes
Major capsid protein of Autographa californica multiple nucleopolyhedrovirus contributes to the promoter activity of the very late viral genes
复制标题
苜蓿银纹夜蛾多核多角体病毒的主要衣壳蛋白有助于非常晚期病毒基因的启动子活性
DOI：
10.1016/j.virusres.2019.197758
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发表时间：
2019-11-01
期刊：
影响因子：
5
通讯作者：
Wang, Yun
中科院分区：
文献类型：
--
作者：
Bai, Huimin;Hu, Yangyang;Wang, Yun
杆状病毒表达载体系统（BEVS）是目前最强大的真核表达系统之一。重组蛋白的表达通常由杆状病毒极晚期基因（即多面蛋白和p10）的启动子控制；因此，确定这些启动子的新调控因子是提高BEVS生产力的关键。加州签名多核多角体病毒（AcMNPV）是BEVS中最常用的病毒载体。VP39是AcMNPV的主要核衣壳蛋白，在细胞核内的核衣壳组装中起着关键作用。在这项研究中，我们发现从AcMNPV基因组中敲除vp39导致多面蛋白和P10蛋白丰度降低。进一步的分析显示，在缺乏vp39的情况下，多面蛋白和p10的mRNA转录本和启动子活性降低，这表明vp39参与了极晚病毒基因启动子的活性，可能是优化当前BEVS的一种手段。
The baculovirus expression vector system (BEVS) is one of the most powerful eukaryotic expression systems. Recombinant protein expression is usually controlled by promoters of the baculovirus very late genes (i.e., polyhedrin and p10); therefore, identifying novel regulatory factors for these promoters is key to increasing BEVS productivity. Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is the viral vector most frequently used in BEVS. VP39 is the major nucleocapsid protein of AcMNPV and plays a pivotal role in nucleocapsid assembly in the nucleus. In this study, we found that knocking out vp39 from the AcMNPV genome resulted in decreased protein abundance of polyhedrin and P10. Further assays revealed that the mRNA transcripts and the promoter activities of polyhedrin and p10 were decreased in the absence of vp39, suggesting that VP39 contributes to the activity of the very late viral gene promoters and may represent a means of optimizing the current BEVS.