Conflicting evidence for HIV enrichment in CD32(+) CD4 T cells.
Conflicting evidence for HIV enrichment in CD32(+) CD4 T cells.
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DOI:
10.1038/s41586-018-0493-4
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发表时间:
2018-09
期刊:
影响因子:
64.8
通讯作者:
Boritz EA
中科院分区:
文献类型:
--
作者:
Pérez L;Anderson J;Chipman J;Thorkelson A;Chun TW;Moir S;Haase AT;Douek DC;Schacker TW;Boritz EA
MethodsParticipant recruitment and informed consent were performed under Institutional Review Board (IRB)-approved protocols at the US National Institutes of Health (NIH). For FACS, whole PBMCs were stained with monoclonal antibodies matching those used by Descours et al. 1 (see Supplementary Methods) and sorted on a BD FACSAria. To evaluate purity, a portion of each population was re-analysed on the flow cytometer after sorting. Virus DNA copies in sorted cells were enumerated by fluorescence-assisted clonal amplification 2. DNA recovery was quantified by albumin (ALB) quantitative PCR. Because the FUN-2 monoclonal antibody used
影响因子:
64.8
作者:
Descours, Benjamin;Petitjean, Gael;Benkirane, Monsef
通讯作者:
Benkirane, Monsef
影响因子:
64.5
作者:
Boritz EA;Darko S;Swaszek L;Wolf G;Wells D;Wu X;Henry AR;Laboune F;Hu J;Ambrozak D;Hughes MS;Hoh R;Casazza JP;Vostal A;Bunis D;Nganou-Makamdop K;Lee JS;Migueles SA;Koup RA;Connors M;Moir S;Schacker T;Maldarelli F;Hughes SH;Deeks SG;Douek DC
通讯作者:
Douek DC