Time course study of aluminum-induced callose formation in barley roots as observed by digital microscopy and low-vacuum scanning electron microscopy
Time course study of aluminum-induced callose formation in barley roots as observed by digital microscopy and low-vacuum scanning electron microscopy
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数字显微镜和低真空扫描电子显微镜观察铝诱导大麦根胼胝质形成的时程研究
DOI：
10.1080/00380768.1999.10415833
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发表时间：
1999
影响因子：
2
通讯作者：
S. Mori
中科院分区：
文献类型：
--
作者：
M. Kaneko;E. Yoshimura;N. Nishizawa;S. Mori
摘要为了阐明AI短期抑制根伸长的机制(S)，我们用数字显微镜对大麦根的形态变化进行了监测。在37µM AI照射后30min内，距根尖1.5 mm处的根表皮表面变得粗糙，并开始出现损伤迹象。38min后，从根冠与根表皮的交界处迅速分泌出大量的愈伤组织，并形成直径约60微米的球形团块。用低真空扫描电子显微镜分析了根表面的精细结构。50min后，远侧0.6 mm处的含量显著增加。同时，根的伸长完全停止。荧光染色结果表明，在细胞伸长区(初生根和根毛的伸长区)的表面有少量的愈伤组织，而在分生组织的表面则没有。根生长减少..。
Abstract To clarify the mechanism(s) involved in the short-term inhibition of root elongation by AI, we monitored the morphological changes of barley roots by digital microscopy. Within 30 min after exposure to 37 µM AI, the surface of the root epidermis in the region of a distance of 1.5 mm from the root tip became rough and began to show signs of damage. After 38 min, callose was rapidly excreted from the junction between the root cap and the root epidermis, and formed a spherical lump approximately 60 µm in diameter. The fine structure of the callose deposits on the root surface was analyzed by low-vacuum scanning electron microscopy. After 50 min, there was a significant increase in the callose contents in the distal 0.6 mm part. At the same time, root elongation stopped completely. Fluorescence staining indicated that callose was localized on the surface of the cell elongation area (the elongation zone of primary roots and root hairs), but not on the surface of the meristem. The root growth reduction...