Cross-Kingdom Analysis of Diversity, Evolutionary History, and Site Selection within the Eukaryotic Macrophage Migration Inhibitory Factor Superfamily
Cross-Kingdom Analysis of Diversity, Evolutionary History, and Site Selection within the Eukaryotic Macrophage Migration Inhibitory Factor Superfamily
复制标题
DOI：
10.3390/genes10100740
复制
发表时间：
2019-09
期刊：
影响因子：
3.5
通讯作者：
C. Michelet;E. Danchin;M. Jaouannet;J. Bernhagen;R. Panstruga;K. Kogel;H. Keller;C. Coustau
中科院分区：
文献类型：
--
作者：
C. Michelet;E. Danchin;M. Jaouannet;J. Bernhagen;R. Panstruga;K. Kogel;H. Keller;C. Coustau
巨噬细胞移动抑制因子（Macrophage migration inhibitory factors，MIF）是一种多功能蛋白质，在哺乳动物体内起着重要的调节作用，包括激活先天性免疫应答。MIF蛋白也在无脊椎生物的先天免疫中发挥作用，或在寄生生物中作为毒力因子，这引起了它们进化史的问题。我们对803种植物、真菌、原生生物和动物的MIF存在或不存在以及进化关系进行了广泛的调查，并探讨了与个别物种的分类地位、生态学和生活方式的潜在关系。我们发现，MIF在真核生物的进化历史是复杂的，涉及可能的祖先复制，多个基因丢失和最近的分支特异性重复。有趣的是，MIFs似乎是必不可少的，并且在一些界域中具有许多纯化选择的位点（例如，植物），而在其他王国中，它们看起来更像植物（例如，在真菌中）或存在于几个分歧的变体中（例如，昆虫，线虫），表明蛋白质超家族内的潜在新功能化。
Macrophage migration inhibitory factors (MIF) are multifunctional proteins regulating major processes in mammals, including activation of innate immune responses. MIF proteins also play a role in innate immunity of invertebrate organisms or serve as virulence factors in parasitic organisms, raising the question of their evolutionary history. We performed a broad survey of MIF presence or absence and evolutionary relationships across 803 species of plants, fungi, protists, and animals, and explored a potential relation with the taxonomic status, the ecology, and the lifestyle of individual species. We show that MIF evolutionary history in eukaryotes is complex, involving probable ancestral duplications, multiple gene losses and recent clade-specific re-duplications. Intriguingly, MIFs seem to be essential and highly conserved with many sites under purifying selection in some kingdoms (e.g., plants), while in other kingdoms they appear more dispensable (e.g., in fungi) or present in several diverged variants (e.g., insects, nematodes), suggesting potential neofunctionalizations within the protein superfamily.