Neonatal superior ovarian nerve transection inhibits follicle development by enhancing follicular atresia and suppressing granulosa cell proliferation in rats.
Neonatal superior ovarian nerve transection inhibits follicle development by enhancing follicular atresia and suppressing granulosa cell proliferation in rats.
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DOI：
10.1071/rd09271
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发表时间：
2010-09
期刊：
影响因子：
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通讯作者：
Xiaoxin Zhang;Lei Zhang;Shuying Huo;Jianlin Wang;S. Cui
中科院分区：
文献类型：
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作者：
Xiaoxin Zhang;Lei Zhang;Shuying Huo;Jianlin Wang;S. Cui
卵巢交感神经参与哺乳动物卵巢功能的调节，但新生卵巢交感神经是否参与卵泡发育及相关机制尚不清楚。本研究于出生后第2天（PD）切断新生大鼠的上级卵巢神经（SON），在PD 30和PD 90观察卵泡发育、卵巢激素分泌、排卵率、颗粒细胞增殖和凋亡。结果表明，SON横断减少卵泡数量和大小，减少促性腺激素诱导的排卵，并增强卵泡闭锁。溴脱氧尿苷（BrdU）和裂解的caspase-3免疫组化染色提供的证据表明，SON横断抑制颗粒细胞增殖，促进颗粒细胞凋亡。此外，SON切断增加血清雌二醇水平，但对血清孕酮水平没有影响。这些结果表明，交感神经对卵巢的供应是重要的卵泡发育和卵巢功能的调节。这些结果对于进一步了解卵巢发育和功能的神经内分泌调节至关重要，但其机制需要在未来的研究中阐明。
The ovarian sympathetic nerves participate in the regulation of mammalian ovarian function, but it is still not known whether the neonatal ovarian sympathetic nerve is involved in follicular development and related mechanisms. In the present study, the superior ovarian nerve (SON) of the neonatal rat was transected on postnatal day (PD) 2, and follicle development, ovarian hormone secretion, ovulation rate, granulosa cell proliferation and apoptosis were analysed on PD 30 and PD 90. The results demonstrate that SON transection decreases follicle number and size, reduces ovulation induced by gonadotrophin and enhances follicular atresia. Bromodeoxyuridine (BrdU) and cleaved caspase-3 immunohistochemistry staining provide evidence that SON transection inhibits granulosa cell proliferation and promotes granulosa cell apoptosis. In addition, SON transection increases serum oestradiol levels, but has no influence on serum progesterone levels. These results suggest that the sympathetic nerve supply to the ovaries is important in regulating follicle development and ovary function. These results are critical for further understanding of the neuroendocrine regulation of ovary development and function, although the mechanism needs to be elucidated in future studies.