DETECTION OF CELLULASE ACTIVITY IN POLYACRYLAMIDE GELS USING CONGO RED-STAINED AGAR REPLICAS
DETECTION OF CELLULASE ACTIVITY IN POLYACRYLAMIDE GELS USING CONGO RED-STAINED AGAR REPLICAS
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DOI：
10.1016/0003-2697(83)90178-1
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发表时间：
1983-01-01
影响因子：
2.9
通讯作者：
BEGUIN, P
中科院分区：
文献类型：
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作者：
BEGUIN, P
在聚丙烯酰胺凝胶电泳后，通过将平板凝胶铺在含有0.1%羧甲基纤维素的2%琼脂薄片上，可以观察到具有纤维素分解活性的条带。在适当的孵育时间后，通过用刚果红对琼脂复制品染色来显示羧甲基纤维素水解区。[使用热纤梭菌]。
Bands that have cellulolytic activity are visualized after polyacrylamide gel electrophoresis by laying the slab gel on top of a thin sheet of 2% agar containing 0.1% carboxymethylcellulose. After a suitable incubation time, zones of carboxymethylcellulose hydrolysis are revealed by staining the agar replica with Congo red. [Clostridium thermocellum was used.].