The SUP35 omnipotent suppressor gene is involved in the maintenance of the non-Mendelian determinant [psi+] in the yeast Saccharomyces cerevisiae.
The SUP35 omnipotent suppressor gene is involved in the maintenance of the non-Mendelian determinant [psi+] in the yeast Saccharomyces cerevisiae.
复制标题
SUP35 全能抑制基因参与酿酒酵母中非孟德尔决定簇[psi]的维持。
DOI：
10.1093/genetics/137.3.671
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发表时间：
1994
期刊：
影响因子：
3.3
通讯作者：
Smirnov,VN
中科院分区：
文献类型：
--
作者：
Ter-Avanesyan,MD;Dagkesamanskaya,AR;Kushnirov,VV;Smirnov,VN
酿酒酵母SUP35基因编码一个76.5-kD的核糖体相关蛋白(Sup35p)，其C端部分与EF-1α延伸因子高度相似，而其N端区域是独一无二的。SUP35基因的突变或过度表达可以产生全能的抑制效应。在目前的研究中，SUP35野生型基因被在体外产生的缺失等位基因所取代，这些缺失等位基因编码的Sup35p缺乏全部或部分独特的N-末端区域。在单倍体菌株中，这些5‘-缺失等位基因同时导致抗抑制作用和非孟德尔决定簇[psi+]的丢失。抗抑制作用是显性的，消除[psi+]决定簇是隐性性状。检测了一组SUP35基因的质粒携带的缺失等位基因保持[psi+]的能力。结果表明，Sup35p的前114个氨基酸足以维持[psi+]决定簇。我们建议Sup35p作为维持[psi+]所需的反式作用因子。
The SUP35 gene of yeast Saccharomyces cerevisiae encodes a 76.5-kD ribosome-associated protein (Sup35p), the C-terminal part of which exhibits a high degree of similarity to EF-1 alpha elongation factor, while its N-terminal region is unique. Mutations in or overexpression of the SUP35 gene can generate an omnipotent suppressor effect. In the present study the SUP35 wild-type gene was replaced with deletion alleles generated in vitro that encode Sup35p lacking all or a part of the unique N-terminal region. These 5'-deletion alleles lead, in a haploid strain, simultaneously to an antisuppressor effect and to loss of the non-Mendelian determinant [psi+]. The antisuppressor effect is dominant while the elimination of the [psi+] determinant is a recessive trait. A set of the plasmid-borne deletion alleles of the SUP35 gene was tested for the ability to maintain [psi+]. It was shown that the first 114 amino acids of Sup35p are sufficient to maintain the [psi+] determinant. We propose that the Sup35p serves as a trans-acting factor required for the maintenance of [psi+].