Functional and evolutionary insights into the simple yet specific gut microbiota of the honey bee from metagenomic analysis
Functional and evolutionary insights into the simple yet specific gut microbiota of the honey bee from metagenomic analysis
复制标题
DOI：
10.4161/gmic.22517
复制
发表时间：
2013-01-01
期刊：
影响因子：
12.2
通讯作者：
Moran, Nancy A.
中科院分区：
文献类型：
--
作者：
Engel, Philipp;Moran, Nancy A.
蜜蜂（Apis mellifera）具有一种特征性的肠道微生物群，仅由少数几种物种组成，这些物种似乎是社会蜜蜂特有的。这种稳定和独特的微生物群落的维持取决于这些昆虫的社会生活方式。与其他动物一样，蜜蜂肠道中的细菌可能对宿主健康至关重要。我们最近对A.意大利蜜蜂，分配基因内容的箱子对应的主要物种存在于蜜蜂肠道，并比较这些物种之间的功能基因类别，并在完整的宏基因组和其他动物之间。基因含量可能与不同的共生功能有关。此外，我们发现这些物种中的每一个都具有高度的遗传多样性。在的情况下，gammaproteobacterial种Gilbertella apicola，我们的实验表明分离株的遗传变异和功能差异之间的联系，这表明该物种内的生态位分区已经出现在进化过程中与其蜜蜂宿主。只有少数几个物种的一致存在，加上这些物种中的每一个物种的菌株变异，使社会蜜蜂的肠道微生物群成为研究宿主相关微生物群落的功能，结构和进化方面的理想模型：许多特征类似于人类和其他哺乳动物的肠道微生物群，但复杂性大大降低。在本附录中，我们总结并讨论了我们的主要发现，并对未来的研究提供了详细的观点。
The honey bee, Apis mellifera, harbors a characteristic gut microbiota composed of only a few species which seem to be specific to social bees. The maintenance of this stable and distinct microbial community depends on the social lifestyle of these insects. As in other animals, the bacteria in the gut of honey bees probably govern important functions critical to host health. We recently sequenced a metagenome of the gut microbiota of A. mellifera, assigned gene contents to bins corresponding to the major species present in the honey bee gut, and compared functional gene categories between these species, and between the complete metagenome and those of other animals. Gene contents could be linked to different symbiotic functions with the host. Further, we found a high degree of genetic diversity within each of these species. In the case of the gammaproteobacterial species Gilliamella apicola, we experimentally showed a link between genetic variation of isolates and functional differences suggesting that niche partitioning within this species has emerged during evolution with its bee hosts. The consistent presence of only a few species, combined with strain variation within each of these species, makes the gut microbiota of social bees an ideal model for studying functional, structural, and evolutionary aspects of host-associated microbial communities: many characteristics resemble the gut microbiota of humans and other mammals, but the complexity is considerably reduced. In this addendum, we summarize and discuss our major findings and provide a detailed perspective on future research.