Single cell mass cytometry reveals remodeling of human T cell phenotypes by varicella zoster virus.
Single cell mass cytometry reveals remodeling of human T cell phenotypes by varicella zoster virus.
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DOI：
10.1016/j.ymeth.2015.07.008
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发表时间：
2015-11-15
期刊：
影响因子：
--
通讯作者：
Arvin AM
中科院分区：
文献类型：
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作者：
Sen N;Mukherjee G;Arvin AM
最近应用于生物学的质量细胞术(CyTOF)提供了一种在单细胞水平上监测多个宿主细胞因子同时变化的“系统”方法。我们用细胞周期图检测了感染水痘带状疱疹病毒(VZV)的T细胞，记录了这种嗜T细胞的人类疱疹病毒引起的病毒介导的表型和功能变化。在这里，我们使用两组互补的针对表面和细胞内信号蛋白的抗体来总结我们的发现，以阐明VZV介导的干扰对受感染T细胞表面和信号网络的影响。用几种统计、分析和可视化工具分析CyTOF数据，包括层次聚类法、正交标度法、SPADE法、ViSNE法和Slide法。大量细胞学研究的数据表明，VZV感染导致T细胞表面结构的“重塑”，促进皮肤转运表型，并伴随着T细胞受体和PI3-激酶通路的激活。这种方法为了解病毒与分化的宿主细胞之间的相互作用提供了一种新的方法，对致病机制非常重要。
The recent application of mass cytometry (CyTOF) to biology provides a ‘systems’ approach to monitor concurrent changes in multiple host cell factors at the single cell level. We used CyTOF to evaluate T cells infected with varicella zoster virus (VZV) infection, documenting virus-mediated phenotypic and functional changes caused by this T cell tropic human herpesvirus. Here we summarize our findings using two complementary panels of antibodies against surface and intracellular signaling proteins to elucidate the consequences of VZV-mediated perturbations on the surface and in signaling networks of infected T cells. CyTOF data was analyzed by several statistical, analytical and visualization tools including hierarchical clustering, orthogonal scaling, SPADE, viSNE, and SLIDE. Data from the mass cytometry studies demonstrated that VZV infection led to ‘remodeling’ of the surface architecture of T cells, promoting skin trafficking phenotypes and associated with concomitant activation of T-cell receptor and PI3-kinase pathways. This method offers a novel approach for understanding viral interactions with differentiated host cells important for pathogenesis.