Overexpression of OrbHLH001, a putative helix-loop-helix transcription factor, causes increased expression of AKT1 and maintains ionic balance under salt stress in rice
Overexpression of OrbHLH001, a putative helix-loop-helix transcription factor, causes increased expression of AKT1 and maintains ionic balance under salt stress in rice
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DOI：
10.1016/j.jplph.2012.08.019
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发表时间：
2013-01-01
影响因子：
4.3
通讯作者：
Xu, Yunyuan
中科院分区：
文献类型：
--
作者：
Chen, Yuan;Li, Fei;Xu, Yunyuan
作为转录因子的基本螺旋-环-螺旋蛋白质家族在植物和动物中得到了广泛的研究。然而，这些因素促进胁迫耐受性的分子机制尚不清楚。本文报道了东乡野生稻OrbHLH 001基因在转基因水稻中的过量表达及其耐盐性。OrbHLH 001的表达具有组织特异性，主要分布在整个植株的韧皮部组织中。离子分析结果表明，NaCl胁迫对OrbHLH 001过表达植株Na+外流和K+内流的影响大于野生型。OrbHLH 001蛋白通过与OsAKT 1启动子区域的E-box基序特异性结合，诱导OsAKT 1的表达，从而调节OsAKT 1在OrbHLH 001过表达植株中的Na+/K+比值。该机制在水稻分子育种中具有潜在的应用价值。(c)2012 Elsevier GmbH. All rights reserved.
The basic helix-loop-helix family of proteins, which function as transcription factors, have been intensively studied in plants and animals. However, the molecular mechanism of these factors contributing to stress tolerance is unknown. Here, We report on the overexpression of OrbHLH001 from Dongxiang wild rice (Oryza rufipogon) conferring salt tolerance in transgenic rice plants. The expression of OrbHLH001 was tissue specific, mainly in phloem tissues throughout the plant. Ion assay with the scanning ion-selective electrode technique showed that NaCl stress has a greater influence on Na+ efflux and K+ influx in OrbHLH001-overexpressed plants than the wild type. OrbHLH001 protein can induce the expression of OsAKT1 to regulate the Na+/K+ ratio in OrbHLH001-overexpressed plants by specifically binding to an E-box motif in the promoter region of OsAKT1. The Mechanism may have potential use in rice molecular breeding. (c) 2012 Elsevier GmbH. All rights reserved.