Rice transgenic plants with suppressed expression of the β subunit of the heterotrimeric G protein
Rice transgenic plants with suppressed expression of the β subunit of the heterotrimeric G protein
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DOI：
10.4161/psb.19378
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发表时间：
2012-04
影响因子：
2.9
通讯作者：
Yuzuko Utsunomiya;Chihiro Samejima;Yukiko Fujisawa;H. Kato;Y. Iwasaki
中科院分区：
文献类型：
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作者：
Yuzuko Utsunomiya;Chihiro Samejima;Yukiko Fujisawa;H. Kato;Y. Iwasaki
水稻异源三聚体G蛋白α亚基基因（RGA1）缺陷突变体d1由于细胞数量减少而表现为矮化和小粒。水稻异源三聚体G蛋白β亚基基因（RGB1）的突变体尚未分离到。为了确定RGB1的功能，采用RNAi方法对RGB1表达抑制的转基因水稻植株进行了研究。RGB1基因敲除线表现为椎板关节区和淋巴结褐变，生育能力下降，但d1中未见这些异常。未获得G蛋白β亚基显著降低的转基因植株，提示RGB1 mRNA的完全抑制可能是致命的。相比之下，d1突变体完全失去G蛋白α亚基，具有可育性，大小为WT的一半。这些研究表明RGB1与RGA1具有不同的功能。
The deficient mutant for the rice heterotrimeric G protein α subunit gene (RGA1), d1, showed dwarfism and set small seed due to a reduced cell number. Mutants for the rice heterotrimeric G protein β subunit gene (RGB1) have not been isolated. To determine the functions of RGB1, transgenic rice plants with suppressed expression of RGB1 were studied using the RNAi method. RGB1 knock-down lines showed browning of the lamina joint regions and nodes and reduced fertility, but these abnormality were not observed in d1. Transgenic plants in which the G protein β subunit was greatly decreased were not obtained, suggesting that the complete suppression of RGB1 mRNA may be lethal. In contrast, the d1 mutants, with complete loss of the G protein α subunit, were fertile and half the size of the WT. These studies suggest that RGB1 has different functions than RGA1.