A Fourier Transform-Induced Data Process for Label-Free Selective Nanopore Analysis under Sinusoidal Voltage Excitations
A Fourier Transform-Induced Data Process for Label-Free Selective Nanopore Analysis under Sinusoidal Voltage Excitations
复制标题
正弦电压激励下无标记选择性纳米孔分析的傅里叶变换诱导数据处理
DOI：
10.1021/acs.analchem.0c01339
复制
发表时间：
2020
影响因子：
7.4
通讯作者：
Lishi Wang
中科院分区：
文献类型：
--
作者：
Xuye Liu;Qiang Zeng;Cheng Liu;Lishi Wang
基于电阻脉冲技术的纳米孔分析是一种有吸引力的单分子检测工具，但它在选择性上存在很大的缺点。应对这一挑战的一个常见解决方案是通过提高分析物脉冲的灵敏度来增加额外的感应适配体和标签。与标记方法相比，我们开发并展示了一种新的无标记纳米孔分析数据过程，该过程可以将电阻电流信号转换为更具体的频率域相角特征，同时使用正弦电压激励和傅里叶变换。特别是，我们发现在正弦电压下纳米颗粒易位引起的跨壁电容在这一过程中起着重要作用，使相角特征更加明显。在实际应用中，该方法成功地通过纳米吸管通过其独特的相位角直接区分了大小相似的SiO2、Ag和Au纳米颗粒以及HeLa和LoVo软生物，甚至在更复杂的SiO2、Ag和Au纳米颗粒混合物的情况下的易位事件。
Nanopore analysis based on a resistive-pulse technique is an attractive tool for single-molecule detection in different fields, but it suffers a great drawback in selectivity. A common solution to this challenge is to add extra sensing aptamers and labels to analytes by improving the sensitivity of their pulses for distinguishing. Compared to the labeling methods, we alternatively develop and demonstrate a novel data process for label-free nanopore analysis that enables the conversion of resistive current signals to more specific frequency domain phase angle features with the contribution from both sinusoidal voltage excitation and Fourier transform. In particular, we find that the transmural capacitance induced by nanoparticle translocations under a sinusoidal voltage plays an important role in this process, making phase angle features more pronounced. In practical applications, the method is successfully applied to directly distinguish the translocation events through a nanopipette by their unique phase angles for similarly sized SiO2, Ag, and Au nanoparticles and soft living organisms of HeLa and LoVo and even in a more complicated case of a SiO2, Ag, and Au nanoparticle mixture.