Diurnal secretion of ghrelin, growth hormone, insulin binding proteins, and prolactin in normal weight and overweight subjects with and without the night eating syndrome
Diurnal secretion of ghrelin, growth hormone, insulin binding proteins, and prolactin in normal weight and overweight subjects with and without the night eating syndrome
复制标题
有或没有夜食综合征的正常体重和超重受试者昼夜分泌的胃泌素、生长激素、胰岛素结合蛋白和催乳素
DOI：
10.1016/j.appet.2012.07.015
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发表时间：
2012-12-01
期刊：
影响因子：
5.4
通讯作者：
Florholmen, Jon R.
中科院分区：
文献类型：
--
作者：
Birketvedt, Grethe S.;Geliebter, Allan;Florholmen, Jon R.
调节肽ghrelin已被提出来帮助调节饥饿和睡眠。夜间进食综合征（内斯）的神经内分泌昼夜节律模式已被区分为褪黑激素和瘦素的血浆浓度的衰减夜间上升和皮质醇的浓度增加较大。在这项研究中，我们想测试的假设，夜猫子有干扰的昼夜水平的生长激素释放肽，生长激素（GH）和相关的调节肽。在12名女性夜间进食者（6名正常体重和6名超重）和25名健康对照者（12名正常体重和13名超重）中，在24小时内采集血液。从早上8点到晚上8点共进餐4次，每隔一小时抽取血样，用放射免疫法（RIA）测定血浆ghrelin浓度和GH。采用ELISA法检测血清GH、IGF-1、IGFBP-3和催乳素。在正常体重的健康受试者中，ghrelin的夜间增加轻微但不显著，而在健康超重、内斯正常体重和内斯超重患者中观察到或多或少的平坦曲线。RMANOVA分析显示超重对生长激素释放肽的总平均值有显著的独立降低作用。未发现对内斯正常体重和超重受试者的直接影响，但发现健康超重和超重内斯受试者之间存在近乎显着的相互作用。超重和内斯对血清GH水平有独立的显著性降低作用。在时间过程中，未观察到IGF-1或IGFB-3的血清水平的变化。超重和内斯对IGF-1水平的独立显著降低作用被检测到，而在两个内斯组中观察到IGFBP-3的总体水平的接近显著降低。最后，在所有四个亚组中观察到血清催乳素水平的显著夜间变化。内斯受试者的总平均水平往往较高，而在健康超重（ns）受试者中观察到相反的情况。我们的结论是，在这两个内斯组和健康超重受试者或多或少衰减ghrelin和GH分泌观察，而不同的分泌观察到催乳素。(C)2012爱思唯尔有限公司保留所有权利。
The regulatory peptide ghrelin has been proposed to help mediate both hunger and sleep. The neuroendocrine circadian patterns in the night eating syndrome (NES) have been distinguished by an attenuated nocturnal rise in the plasma concentrations of melatonin and leptin and a greater increase in the concentrations of cortisol. In this study we wanted to test the hypothesis that night eaters have disturbances in the circadian levels of ghrelin, growth hormone (GH) and associated regulatory peptides. In 12 female night eaters (6 normal weight and 6 overweight), and 25 healthy controls (12 normal weight and 13 overweight), blood was sampled over a 24-hour period. Four meals were served from 8 AM to 8 PM, and blood samples were drawn every second hour for determination of plasma ghrelin concentrations and GH by radioimmunoassay (RIA). Analysis of serum GH, IGF-1, IGFBP-3 and prolactin were performed by ELISA. In healthy normal weight subjects there was a slight but non significant nocturnal increase of ghrelin, whereas a more or less flat curve was observed for healthy overweight, NES normal weight and NES overweight patients. The RMANOVA analysis showed a significant independent lowering effect of overweight on the grand mean of ghrelin. No direct effects on NES normal weight and overweight subjects were found, but a near-significant interaction was found between healthy overweight and overweight NES subjects. There were independent significant lowering effects of overweight and NES on the serum GH levels. During the time course no changes in the serum levels of IGF-1 or IGFB-3 were observed. Independent significant lowering effects of overweight and NES on the levels of IGF-1 were detected, whereas a near significant reduction in the global levels of IGFBP-3 was observed in both NES groups. Finally, significant nocturnal changes were observed for serum levels of prolactin in all four subgroups. Grand mean levels tended to be higher in NES subjects whereas the opposite was observed in healthy overweight (ns). We conclude that in both NES groups and in healthy overweight subjects more or less attenuated ghrelin and GH secretions were observed, whereas divergent secretions were observed for prolactin. (C) 2012 Elsevier Ltd. All rights reserved.