Tissue Culture Studies on Murine Virus-induced Leukemia Cells: Isolation of Single Cells in Agar-Liquid Medium
Tissue Culture Studies on Murine Virus-induced Leukemia Cells: Isolation of Single Cells in Agar-Liquid Medium
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鼠病毒诱导的白血病细胞的组织培养研究：琼脂液体培养基中单细胞的分离
DOI：
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发表时间：
1967
期刊：
影响因子：
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通讯作者：
C. Friend
中科院分区：
文献类型：
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作者：
M. C. Patuleia;C. Friend
在小鼠病毒诱导的白血病细胞的长期细胞培养中观察到沿红细胞线的成熟。为了确定这些培养中不同形态类型之间的关系，设计了一种利用琼脂液体培养基的桑德斯电镀和克隆细胞技术。这种方法使单细胞克隆成为可能。每个克隆的后代由原始细胞、原红细胞和红母细胞组成。从这些数据来看，我们认为所研究的小鼠白血病的原始细胞特征可能是红细胞前体。
Summary Maturation along the erythrocytic line is observed in long-term cell cultures of murine virus-induced leukemic cells. In attempts to determine the relationship between the different morphologic types in these cultures, an adaptation of the Sanders technic of plating and cloning cells utilizing an agar-liquid medium was devised. This method permitted the cloning of single cells. The progeny of each clone consisted of primitive cells, proerythroblasts, and erythroblasts. From these data the possibility is considered that the primitive cell characteristic of the murine leukemia under study is an erythrocyte precursor.