Selected DNA aptamers as hydroxyapatite affinity reagents
Selected DNA aptamers as hydroxyapatite affinity reagents
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DOI：
10.1016/j.aca.2020.03.029
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发表时间：
2020-05-08
影响因子：
6.2
通讯作者：
Gerdon, Aren E.
中科院分区：
文献类型：
--
作者：
Duffy, Emma;Florek, John;Gerdon, Aren E.
选择DNA适体是因为它们能够特异性和快速地结合结晶羟基磷灰石(Ca-10(PO4)(6)(OH)(2)；是牙釉质和骨骼的主要矿物成分。核酸适配体的g -核苷酸比例增加，并倾向于形成g -四重体二级结构。与对照序列相比，其中一个适体具有高亲和力和高负载能力，具有增强的结合动力学，并且对结晶HAP材料具有特异性，而不是无定形磷酸钙（ACP）和β -磷酸三钙（TCP）。荧光功能化的适体在表面结合实验中被证明可以特异性标记HAP，这表明该适体在需要标记特定磷酸钙材料的生物医学或生物技术领域是有用的。(C) 2020 Elsevier B.V.版权所有
DNA aptamers were selected for their ability to bind specifically and quickly to crystalline hydroxyapatite (Ca-10(PO4)(6)(OH)(2); HAP), the primary mineral component of enamel and bone. Aptamers were found to have an enhanced percent of G-nucleotides and a propensity for forming a G-quadruplex secondary structure. One aptamer was studied in comparison to control sequences and was found to bind with high affinity and at high loading capacity, with enhanced binding kinetics, and with specificity for crystalline HAP material over amorphous calcium phosphate (ACP) and beta-tricalcium phosphate (TCP). The fluorescently-functionalized aptamer was demonstrated to specifically label HAP in a surface binding experiment and suggests the usefulness of this selected aptamer in biomedical or biotechnology fields where the labeling of specific calcium phosphate materials is required. (C) 2020 Elsevier B.V. All rights reserved.