Nanofat-derived stem cells with platelet-rich fibrin improve facial contour remodeling and skin rejuvenation after autologous structural fat transplantation.
Nanofat-derived stem cells with platelet-rich fibrin improve facial contour remodeling and skin rejuvenation after autologous structural fat transplantation.
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纳米脂肪干细胞与富含血小板的纤维蛋白可改善自体结构脂肪移植后的面部轮廓重塑和皮肤年轻化
DOI：
10.18632/oncotarget.19721
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发表时间：
2017-09-15
期刊：
影响因子：
--
通讯作者：
Li HM
中科院分区：
文献类型：
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作者：
Wei H;Gu SX;Liang YD;Liang ZJ;Chen H;Zhu MG;Xu FT;He N;Wei XJ;Li HM
传统的自体脂肪移植是治疗面部软组织凹陷和皮肤老化的常用手术方法。然而，移植的脂肪很容易被吸收，降低了手术的长期疗效。在这里，我们检查了纳米脂肪辅助自体脂肪结构移植的疗效。分离、机械乳化、培养和表征纳米脂肪来源的干细胞（NFSC）。富血小板纤维蛋白（PRF）在体外促进NFSCs的增殖和成脂分化。然后，我们比较了62例接受纳米脂肪、PRF和自体脂肪结构移植联合治疗的软组织凹陷或衰老迹象的试验组患者与接受传统自体脂肪移植的对照组患者（77例）。与传统移植相比，纳米脂肪移植后面部软组织凹陷症状和皮肤纹理得到了更大程度的改善，纳米脂肪组的总体满意率超过90%。这些数据表明，NFSC的功能与间充质干细胞相似，并且具有传统脂肪干细胞培养物的许多生物学特征。因此，将联合收割机新分离的纳米脂肪（具有丰富的基质血管成分（SVF））与PRF和自体结构脂肪颗粒相结合的移植可能是重塑面部轮廓和恢复皮肤的安全，高效和持久的方法。
Traditional autologous fat transplantation is a common surgical procedure for treating facial soft tissue depression and skin aging. However, the transplanted fat is easily absorbed, reducing the long-term efficacy of the procedure. Here, we examined the efficacy of nanofat-assisted autologous fat structural transplantation. Nanofat-derived stem cells (NFSCs) were isolated, mechanically emulsified, cultured, and characterized. Platelet-rich fibrin (PRF) enhanced proliferation and adipogenic differentiation of NFSCs in vitro. We then compared 62 test group patients with soft tissue depression or signs of aging who underwent combined nanofat, PRF, and autologous fat structural transplantation to control patients (77 cases) who underwent traditional autologous fat transplantation. Facial soft tissue depression symptoms and skin texture were improved to a greater extent after nanofat transplants than after traditional transplants, and the nanofat group had an overall satisfaction rate above 90%. These data suggest that NFSCs function similarly to mesenchymal stem cells and share many of the biological characteristics of traditional fat stem cell cultures. Transplants that combine newly-isolated nanofat, which has a rich stromal vascular fraction (SVF), with PRF and autologous structural fat granules may therefore be a safe, highly-effective, and long-lasting method for remodeling facial contours and rejuvenating the skin.