Global genome diversity of the Leishmania donovani complex
Global genome diversity of the Leishmania donovani complex
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DOI：
10.7554/elife.51243
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发表时间：
2020-03-25
期刊：
影响因子：
7.7
通讯作者：
Cotton, James A.
中科院分区：
文献类型：
--
作者：
Franssen, Susanne U.;Durrant, Caroline;Cotton, James A.
杜氏利什曼原虫复合体- L. donovani和L.婴儿-导致致命的疾病内脏利什曼病。我们提出了第一个全面的全基因组全球研究，151个培养的外地分离代表大部分的地理分布。L. donovani分离物分为五个组，这些组在很大程度上与地理来源一致，但在多样性上差异很大。相反，大多数L.婴儿样本属于一个全球分布的群体，几乎没有多样性。这幅图因几个杂交谱系而变得复杂。所鉴定的遗传组在杂合性和连锁水平上各不相同，表明不同的重组历史。我们研究了染色体特异性的非整倍体模式，并鉴定了广泛的结构变异，包括已知和疑似的耐药基因座。这项研究揭示了更大的遗传多样性比所建议的地理为重点的研究，为未来的工作提供了基因组变异的资源，并设置场景的进化和遗传学的杜氏利什曼原虫复杂的新的理解。
Protozoan parasites of the Leishmania donovani complex - L. donovani and L. infantum - cause the fatal disease visceral leishmaniasis. We present the first comprehensive genome-wide global study, with 151 cultured field isolates representing most of the geographical distribution. L. donovani isolates separated into five groups that largely coincide with geographical origin but vary greatly in diversity. In contrast, the majority of L. infantum samples fell into one globally-distributed group with little diversity. This picture is complicated by several hybrid lineages. Identified genetic groups vary in heterozygosity and levels of linkage, suggesting different recombination histories. We characterise chromosome-specific patterns of aneuploidy and identified extensive structural variation, including known and suspected drug resistance loci. This study reveals greater genetic diversity than suggested by geographically-focused studies, provides a resource of genomic variation for future work and sets the scene for a new understanding of the evolution and genetics of the Leishmania donovani complex.