Comparison of gene expression in bovine aortic endothelium in vivo versus in vitro. Differences in growth regulatory molecules.
Comparison of gene expression in bovine aortic endothelium in vivo versus in vitro. Differences in growth regulatory molecules.
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体内与体外牛主动脉内皮基因表达的比较。
DOI：
10.1161/01.atv.13.7.985
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Schwartz,SM
中科院分区：
文献类型：
--
作者：
Liaw,L;Schwartz,SM
许多内皮研究都假设培养的汇合细胞和体内内皮层之间是等效的。我们比较了培养物中牛主动脉内皮细胞 (BAEC) 与新鲜分离的牛主动脉细胞的基因表达。通过免疫细胞化学和 mRNA 分析，我们收获体内组织的技术产生了内皮细胞，其标准是含有冯维勒布兰德因子 (vWF) 且缺乏平滑肌 α-肌动蛋白。我们发现，当 BAEC 培养时，一些基因会过度表达，包括碱性成纤维细胞生长因子、血小板衍生生长因子 B 链和血小板反应蛋白。另一方面，vWF 的信息在体内高度表达，而在汇合培养物中表达水平较低。转化生长因子-β、plakoglobin 和 flg（fms 样基因，FGF 受体-1）的转录本在体外和体内具有可比性。这些结果证明内皮细胞从体内条件向组织培养的转变中发生基因表达的显着变化。体外内皮的研究可能很难反映体内静止的内皮衬里，但可能更类似于对损伤或血管生成刺激做出反应的细胞。
Many studies of the endothelium have assumed equivalence between cultured confluent cells and an endothelial lining in vivo. We compared gene expression of bovine aortic endothelial cells (BAECs) in culture versus freshly isolated cells from bovine aortas. Our technique of harvesting in vivo tissue yielded cells that were endothelial by the criteria of their containing von Willebrand factor (vWF) and lacking smooth muscle alpha-actin, by both immunocytochemistry and mRNA analyses. We found that several genes are overexpressed when BAECs are placed into culture, including basic fibroblast growth factor, platelet-derived growth factor B-chain, and thrombospondin. On the other hand, message for vWF is highly expressed in vivo and at lower levels in confluent cultures. The transcripts for transforming growth factor-beta, plakoglobin, and flg (fms-like gene, FGF receptor-1) are comparable in vitro and in vivo. These results demonstrate that significant changes in gene expression occur in the transition from in vivo conditions to tissue culture of endothelial cells. Studies of in vitro endothelium may poorly reflect a quiescent endothelial lining in vivo but may be more similar to cells responding to injury or angiogenic stimuli.