Genetic characterization of Chromobacterium isolates from black water environments in the Brazilian Amazon
Genetic characterization of Chromobacterium isolates from black water environments in the Brazilian Amazon
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DOI：
10.1111/j.1472-765x.2005.01724.x
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发表时间：
2005-01-01
影响因子：
2.4
通讯作者：
Vasconcelos, ATR
中科院分区：
文献类型：
--
作者：
Hungria, M;Astolfi, S;Vasconcelos, ATR
目的：分离并鉴定巴西亚马逊地区堇菜色杆菌的多样性。方法与结果：从巴西亚马逊河内格罗河的水域和河岸分离得到22株菌株。所有分离株均能在44℃和pH 4.0的条件下体外生长，但在低于15℃的条件下会受到不利影响，在4℃条件下无法存活，这些特性可能与对生态系统的适应有关。在rep-PCR分析中，分离株的最终相似度仅为13%。16S rRNA基因分析结果显示，3个主要类群的最终相似度为97%，只有3个分离株与型菌株聚类。23S rRNA基因也获得了类似的数据。结论：高水平的遗传多样性得到了证实，有迹象表明，巴西分离株除了紫紫葡萄属外，至少适合两个新的聚类。研究的意义和影响：结果表明，在亚马逊地区，堇菜（C. violaceum）具有显著的细菌适应性和遗传多样性。
Aims: To isolate and to characterize the diversity of Chromobacterium violaceum from the Brazilian Amazon region.Methods and Results: Twenty-two isolates were obtained from the waters and banks of the river Negro, in the Brazilian Amazon. All isolates were able to grow in vitro at 44 degrees C and pH 4.0, but were adversely affected by temperatures below 15 degrees C, and unable to survive at 4 degrees C, properties that may be related to the adaptation to the ecosystem. The isolates were joined at a final level of similarity of only 13% in the rep-PCR analysis. The analysis of 16S rRNA genes resulted in three main groups clustered at a final level of similarity of 97% and only three isolates were clustered with the type strain. Similar data were obtained for the 23S rRNA gene.Conclusions: A high level of genetic diversity was verified with indications that the Brazilian isolates would fit into at least two new clusters besides C. violaceum species.Significance and Impact of the Study: The results show remarkable bacterial adaptability and genetic diversity of C. violaceum in the Amazon region.