Human olfactory mesenchymal stromal cell transplants promote remyelination and earlier improvement in gait co-ordination after spinal cord injury.
Human olfactory mesenchymal stromal cell transplants promote remyelination and earlier improvement in gait co-ordination after spinal cord injury.
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作者：
Lindsay SL;Toft A;Griffin J;M M Emraja A;Barnett SC;Riddell JS
自体细胞移植是修复受损脊髓的一种有前途的策略。在这里，我们研究了从人嗅粘膜分离的间充质基质细胞移植到不完全脊髓损伤的啮齿动物模型后的修复潜力。外周型髓鞘再生的损伤部位使用免疫细胞化学P0的调查，显示更广泛的分布在移植与对照动物相比。除了在背柱中的典型分布（所有动物共有）外，仅在移植动物中，在损伤部位外侧和腹侧的白色物质中始终检测到P0免疫标记。移植的动物也表现出减少的空化。几项功能结局指标，包括背柱传导的终点电生理学测试和BBB的每周行为测试、负重和疼痛，显示移植动物和对照动物之间无差异。然而，步态分析显示，移植动物的前肢和后肢步进之间的协调恢复较早。这种步态的改善可能与腹侧和外侧白色物质中髓鞘形成的增强有关，这些物质中纤维束对运动很重要。因此，从嗅粘膜自体移植间充质基质细胞可能是有益的治疗脊髓损伤。GLIA 2017 GLIA 2017;65：639-656
Autologous cell transplantation is a promising strategy for repair of the injured spinal cord. Here we have studied the repair potential of mesenchymal stromal cells isolated from the human olfactory mucosa after transplantation into a rodent model of incomplete spinal cord injury. Investigation of peripheral type remyelination at the injury site using immunocytochemistry for P0, showed a more extensive distribution in transplanted compared with control animals. In addition to the typical distribution in the dorsal columns (common to all animals), in transplanted animals only, P0 immunolabelling was consistently detected in white matter lateral and ventral to the injury site. Transplanted animals also showed reduced cavitation. Several functional outcome measures including end‐point electrophysiological testing of dorsal column conduction and weekly behavioural testing of BBB, weight bearing and pain, showed no difference between transplanted and control animals. However, gait analysis revealed an earlier recovery of co‐ordination between forelimb and hindlimb stepping in transplanted animals. This improvement in gait may be associated with the enhanced myelination in ventral and lateral white matter, where fibre tracts important for locomotion reside. Autologous transplantation of mesenchymal stromal cells from the olfactory mucosa may therefore be therapeutically beneficial in the treatment of spinal cord injury. GLIA 2017 GLIA 2017;65:639–656