Identification of Lactobacillus brevis using a species-specific AFLP-derived marker.
Identification of Lactobacillus brevis using a species-specific AFLP-derived marker.
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使用物种特异性 AFLP 衍生标记鉴定短乳杆菌。
DOI：
10.1016/j.ijfoodmicro.2016.06.002
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发表时间：
2016
影响因子：
5.4
通讯作者：
C. Franz
中科院分区：
文献类型：
--
作者：
Vincenzina Fusco;G. M. Quero;Daniele Chieffi;C. Franz
开发了一种简单、特异的快速检测和鉴定短乳杆菌的方法。 L 的 fAFLP（荧光扩增片段长度多态性）标记。 brevis 用于设计用于物种特异性 PCR 测定的寡核苷酸引物，靶向编码 L 的二酮古洛糖酸还原酶家族的醛/酮还原酶的基因的 125 bp 片段。短。该测定导致菌株分配到物种 L 的 100% 包容性和排他性。短。该测定法的分析特异性已成功测试以识别 L.从酸面团中分离出短链。
A simple and specific method for the rapid detection and identification ofLactobacillus breviswas developed. A fAFLP (Fluorescent Amplified Fragment Length Polymorphisms) marker forL. breviswas used to design oligonucleotide primers for a species-specific PCR assay, targeting a 125 bp fragment of the gene encoding the aldo/keto reductase of the diketogulonate-reductase family ofL. brevis. This assay resulted in 100% inclusivity and exclusivity of assignment of strains to the speciesL. brevis. The analytical specificity of this assay was successfully tested to identifyL. brevisisolates from sourdoughs.