Immunohistochemical localization and quantification of glucose transporters in the mouse brain
Immunohistochemical localization and quantification of glucose transporters in the mouse brain
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DOI：
10.1016/s0306-4522(01)00619-4
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发表时间：
2002-01-01
期刊：
影响因子：
3.3
通讯作者：
Messier, C
中科院分区：
文献类型：
--
作者：
Choeiri, C;Staines, W;Messier, C
一个由7个促进葡萄糖转运体(GLUT 1-5、7和8)组成的家族介导了细胞对葡萄糖的摄取。在大脑中，Glut2、Glut5和GLUT8的水平相对较低，而据报道，Glut1、GLUT3和GLUT4在大脑的几个区域中含量丰富。本研究利用免疫荧光技术，对CD1小鼠大脑中主要葡萄糖转运蛋白Glut1、GLUT3和GLUT4在不同脑区的定位进行了研究、比较和定量。小鼠脑内Glut1、GLUT3和GLUT4的染色结果与大鼠的观察结果基本一致。结果证实了GLUT_3在大脑皮层神经纤维中的分布，该转运蛋白在海马苔藓纤维区的表达，以及在海马锥体细胞层的GLUT_3和GLUT_4免疫染色。本研究还报道了转运蛋白的新定位，如GLUT3在神经元核周的存在，GLUT4标记的神经元在海马CA3和下丘脑。在小脑，GLUT3在靠近轴突的浦肯野细胞体的底部显示亚细胞定位。此外，在小脑的颗粒细胞层中，发现一组重要的高尔基细胞对GLUT4有很强的免疫染色。量化结果表明，额叶和运动皮质中Glut1的相对丰度与这些脑区的高能量需求很好地吻合。然而，大脑运动区的GLUT4选择性丰富支持其在提供控制运动活动所需的能量方面的作用。已报道的GLUT3在神经细胞中的分布似乎支持其在突触能量供应和神经递质合成中的作用。我们得出结论，啮齿动物脑中葡萄糖转运体的细胞和区域分布似乎与其相应的功能有关。(C)2002年IBRO。爱思唯尔科学有限公司出版。版权所有。
A family of seven facilitative glucose transporters (Glut 1-5, 7 and 8) mediates the cellular uptake of glucose. In the brain, Glut2, Glut5 and Glut8 are found at relatively low levels whereas Glut1, Glut3 and Glut4 were reported in abundance in several brain regions. Using immunofluorescence, this study investigated, compared and quantified the localization of the brain major glucose transporters, Glut1, Glut3 and Glut4, in the different cerebral areas of CD1 mice. Most of the staining of Glut1, Glut3 and Glut4 in the mouse brain coincides with observations made in rats. The results confirm the cortical neuropil distribution of Glut3, the prominence of this transporter in the mossy fiber field of the hippocampus and the Glut3 and Glut4 immunostaining of the hippocampal pyramidal cell layer. The present study also reports novel localizations of the transporters such as the presence of Glut3 in neuronal perikarya, Glut4-labeled neurons in the CA3 of the hippocampus and the subiculum. In the cerebellum, Glut3 shows subcellular localization to the base of the Purkinje cell bodies near the axon hillock. Furthermore, an important population of Golgi cells was found to be strongly immunostained for Glut4 in the granular cell layer of the cerebellum. The quantification results suggest that the relative abundance of Glut1 in the frontal and motor cortices coincides well with the high-energy demands of these brain regions. However, the Glut4-selective abundance in cerebral motor areas supports its suggested role in providing the energy needed for the control of the motor activity. The reported neuropil distribution of Glut3 seems to uphold its suggested role in synaptic energy provision and neurotransmitter synthesis.We conclude that the cellular and regional distributions of the glucose transporters in the rodent brain seem to be relevant to their corresponding functions. (C) 2002 IBRO. Published by Elsevier Science Ltd. All rights reserved.