Comparison of the comet assay and the oxygen microelectrode for measuring tumor oxygenation in head-and-neck cancer patients
Comparison of the comet assay and the oxygen microelectrode for measuring tumor oxygenation in head-and-neck cancer patients
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DOI：
10.1016/s0360-3016(02)04503-0
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发表时间：
2003-06-01
影响因子：
7
通讯作者：
Brown, JM
中科院分区：
文献类型：
--
作者：
Le, QT;Kovacs, MS;Brown, JM
目的：比较Eppendorf Po-2组织图和碱性彗星试验在头颈部鳞癌患者肿瘤乏氧检测中的应用。材料与方法：65例头颈部鳞状细胞癌淋巴结转移患者在5Gy1分钟和2分钟分别进行组织学和彗星实验。54名患者在1分钟有足够的肿瘤细胞用于彗星分析，26名患者在1分钟和2分钟都有足够的肿瘤细胞进行分析。用碱性凝胶电泳法检测单个细胞的DNA单链断裂，并用中位数尾矩(MTM)来量化这些值的分布。结果：1分钟和2分钟的MTM显著相关(斜率=0.77+/-0.03)。体内和体外照射的肿瘤细胞DNA损伤之间没有相关性。Eppendorf Po-2测量结果与彗星MTM之间没有发现相关性。研究结节的治疗反应与彗星MTMS之间有统计学意义的相关性，而治疗反应与艾彭多夫测量之间没有相关性。结论：彗星试验是可重复性的，从1分钟和2分钟的活检中可以看出。MTM的肿瘤间变异不是内在放射敏感性的结果，而是肿瘤缺氧的结果。Eppendorf Po-2测量结果与彗星MTM之间没有相关性。在预测治疗反应作为短期结果的终点方面，彗星试验比埃彭多夫更好。需要更长时间的随访才能确定彗星试验作为局部肿瘤控制和生存的预测因子的作用。(C)2003年爱思唯尔公司。
Purpose: To compare the Eppendorf Po-2 histograph and the alkaline comet assay as methods of measuring tumor hypoxia in patients with head-and-neck squamous cell carcinomas.Materials and Methods: As part of a larger clinical trial, 65 patients with head-and-neck squamous cell carcinoma nodal metastasis underwent tumor oxygenation measurements with Eppendorf Po-2 histographs and comet assays, performed on fine-needle aspirates at 1 and 2 min after 5 Gy. Fifty-four patients had sufficient tumor cells for comet analysis at 1 min and 26 at both 1 and 2 min. Individual cells were examined for DNA single-strand breaks by alkaline gel electrophoresis, and the distribution of values was quantified using median tail moment (MTM). Nonirradiated tumor cells from pretreatment fine-needle aspirates received 5 Gy in vitro to establish the oxygenated response.Results: There was a significant correlation between the 1- and 2-min MTM (slope = 0.77 +/- 0.03). There was no relationship between DNA damage in tumor cells irradiated in vitro and in vivo. No correlation was found between Eppendorf Po-2 measurements and comet MTM. There was a statistically significant correlation between the treatment response in the node studied and comet MTMs, whereas no correlation was observed between treatment response and Eppendorf measurements.Conclusion: Comet assays are reproducible, as shown by biopsies at 1 and 2 min. Intertumor variation in the MTM is not a result of intrinsic radiosensitivity but of tumor hypoxia. There was no correlation between Eppendorf Po-2 measurements and comet MTM. Comet assays were better than Eppendorf in predicting treatment response as an end point for short-term outcome. Longer follow-up is needed to determine the role of the comet assay as a predictor for locoregional tumor control and survivals. (C) 2003 Elsevier Inc.