RE-FORMATION OF MICROTUBULES IN CLOSTERIUM-EHRENBERGII MENEGHINI AFTER COLD-INDUCED DEPOLYMERIZATION
RE-FORMATION OF MICROTUBULES IN CLOSTERIUM-EHRENBERGII MENEGHINI AFTER COLD-INDUCED DEPOLYMERIZATION
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DOI：
10.1007/bf00391218
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发表时间：
1986-01-01
期刊：
影响因子：
4.3
通讯作者：
HOGETSU, T
中科院分区：
文献类型：
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作者：
HOGETSU, T
用免疫荧光显微镜观察了冷诱导解聚后，埃伦新月藻微管（MT）的重新形成。梭在每日光-暗循环的黑暗期，Ehrenbertus细胞经历细胞分裂，随后是半细胞扩增。在细胞分裂过程中和分裂结束后，有5种类型的MT依次出现和消失，即MT环、毛状MT、纺锤形MT、放射状MT和转移壁MT。壁MT仅在扩展的新半细胞中横向分布。当细胞在冰水中冷却时，扩展细胞中的壁MT被破碎，然后在5分钟内与其它类型的MT一样消失。C冷却2 h后，壁MT和其他类型的MT重新形成。在扩张细胞壁MT重组的早期阶段，形成小的星形MT，然后在扩张的新的和旧的半细胞中随机取向的MT。MT环也在新老半胞的边界处重新形成。在随机排列中没有明显的MT组织中心。随着时间的推移，旧半细胞中随机取向的壁MT消失，而扩展的新半细胞中的壁MT逐渐呈现横向取向。这些结果表明，壁MT可以横向重新排列后，他们已经重新形成，壁MT的成核是从有序的机制分离。
Immunofluorescence microscopy was used to examine the re-formation of microtubules (MT), after cold-induced depolymerization, in Closterium ehrenbergii. The C. ehrenbergii cells undergo cell division followed by semicell expansion in the dark period of daily light-dark cycles. Five types of MTs, namely the MT ring, hair-like MTs around the nuclei, spindle MTs, radially arranged MTs and transferse wall MTs, appeared and disappeared sequentially during and following cell division. The wall MTs were distributed transversely only in the expanding new semicells. When cells were chilled in ice water, wall MTs in expanding cells were fragmented, and then disappeared as did the other types of MTs, within 5 min. When cells were warmed at 20.degree. C after 2 h chilling, wall MTs and the other types of MTs re-formed. At the early stage of wall-MT re-formation in expanding cells, small, star-like MTs were formed, and then randomly oriented MTs developed in both the expanding new and the old semicells. The MT ring was also re-formed at the boundary between the new and old semicells. There were no obvious MT-organizing centers in the random arrangement. As time passed, the randomly oriented wall MTs in the old semicells disappeared and those in the expanding new semicells gradually assumed a transverse orientation. These results indicate that wall MTs can be rearranged transversely after they have been re-formed and that nucleation of wall MTs is separable from the mechanism for ordering them.