ISFET and Dex-AgNPs Based Portable Sensor for Reusable and Real-time Determinations of Concanavalin A and Glucose on Smartphone
ISFET and Dex-AgNPs Based Portable Sensor for Reusable and Real-time Determinations of Concanavalin A and Glucose on Smartphone
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基于 ISFET 和 Dex-AgNP 的便携式传感器，可在智能手机上重复使用并实时测定伴刀豆球蛋白 A 和葡萄糖
DOI：
10.1016/j.bios.2019.111962
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发表时间：
2020
影响因子：
12.6
通讯作者：
Zhao Yue
中科院分区：
文献类型：
--
作者：
Shuang Zhao;Cong Shi;Hongyang Hu;Zhengping Li;Gang Xiao;Qiaochun Yang;Peng Sun;Linyang Cheng;Wencheng Niu;Jinshun Bi;Zhao Yue
在本文中，一个便携式的实时传感装置，建立了刀豆球蛋白A（Con A）和葡萄糖检测使用智能手机。离子敏感场效应晶体管（ISFET）在低工作点的功能被选为一个小尺寸，低功耗的换能器，和葡聚糖封端的银纳米粒子（Dex-AgNPs）作为敏感的纳米探针上的ISFET栅极。利用Con A和碳水化合物之间的亲和力，可以捕获Con A，从而直接通过ISFET/Dex-AgNP单元检测;然后可以通过与葡聚糖竞争从ISFET/Dex-AgNP/Con A单元中去除Con A来间接测定葡萄糖。这种竞争机制对传感器的损害较小，允许传感装置的可重复使用性，并克服了FET装置在盐溶液中的德拜屏蔽。该手持式设备由纽扣电池供电，可达到出色的Con A（0.16 ng mL−1）和葡萄糖（10 nM）检测限，实际上可使用至少20天。
In this paper, a portable real-time sensing device was built for Concanavalin A (Con A) and glucose detection using a smartphone. The ion-sensitive field-effect transistor (ISFET) functioning at a low working point was selected as a small-size, low-power transducer, and dextran-capped silver nanoparticles (Dex-AgNPs) served as sensitive nanoprobes on the ISFET gate. Using the affinity between Con A and carbohydrates, Con A can be captured, and thus directly detected by the ISFET/Dex-AgNPs unit; then glucose can be determined indirectly by removing Con A from the ISFET/Dex-AgNPs/Con A unit via competition with dextran. The mechanism of this competition does less harm to the sensor, allows the reusability of the sensing device, and overcomes the Debye screening of the FET device in saline solutions. Powered by a button cell, the handheld device attains excellent Con A (0.16 ng mL−1) and glucose (10 nM) detection limit, and can practically be used for at least 20 days.