Studies of a human T lymphocyte antigen recognized by a monoclonal antibody.
Studies of a human T lymphocyte antigen recognized by a monoclonal antibody.
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研究单克隆抗体识别的人类 T 淋巴细胞抗原。
DOI：
10.1073/pnas.78.3.1791
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发表时间：
1981
影响因子：
11.1
通讯作者：
Levy,R
中科院分区：
文献类型：
--
作者：
Engleman,EG;Warnke,R;Fox,RI;Dilley,J;Benike,CJ;Levy,R
一种单克隆抗体（命名为L17F12）可以检测95-100%存在于人类外周T淋巴细胞、大多数胸腺细胞和急性淋巴细胞白血病T细胞上的抗原，但不包括B细胞、B细胞系或单核细胞。免疫过氧化物酶法检查冷冻组织切片显示，表达该抗原的细胞主要存在于胸腺髓质和淋巴结和脾脏的t细胞区。L17F12识别的抗原与67,000道尔顿的细胞表面糖蛋白相关。用L17F12从人胸腺细胞、正常外周T细胞、白血病T细胞和T细胞系中分离出该分子。这种抗原在正常T细胞上的表达不因长时间暴露于体外各种T细胞刺激而减少。在缺乏补体的情况下，L17F12与T细胞结合而不改变增殖功能，从而能够快速纯化功能完整的T细胞。在补体存在的情况下，L17F12对T细胞具有溶细胞作用，为从异质群体中消耗T细胞提供了基础。这些数据表明，单克隆抗体L17F12识别特定的t细胞分化蛋白。这种抗体在人体免疫系统的研究中将是有用的。
A monoclonal antibody (designated L17F12) detects an antigen present on 95-100% of human peripheral T lymphocytes, the majority of thymocytes, and acute lymphocytic leukemia T cells but not B cells, B-cell lines, or monocytes. Examination of frozen tissue sections by the immunoperoxidase method revealed that the cells expressing this antigen were found predominantly in the medulla of thymus and in T-cell zones of lymph node and spleen. The antigen recognized by L17F12 was associated with a cell-surface glycoprotein of 67,000 daltons. L17F12 was used to isolate this molecule from human thymocytes, normal peripheral T cells, leukemic T cells, and T-cell lines. Expression of this antigen on normal T cells was not diminished by prolonged exposure in vitro to various T-cell stimuli. In the absence of complement, L17F12 bound to T cells without altering proliferative functions, thus enabling rapid purification of functionally intact T cells. In the presence of complement, L17F12 was cytolytic for T cells, providing the basis for depletion of T cells from heterogeneous populations. These data suggest that the monoclonal antibody L17F12 recognizes a specific T-cell differentiation protein. This antibody will be useful in studies of the human immune system.