The nuclear appearance of ERK1/2 and p38 determines the sequential induction of ATF2-Thr71 and ATF2-Thr69 phosphorylation by serum in JNK-deficient cells
The nuclear appearance of ERK1/2 and p38 determines the sequential induction of ATF2-Thr71 and ATF2-Thr69 phosphorylation by serum in JNK-deficient cells
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DOI：
10.1016/j.mce.2009.07.023
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发表时间：
2009-11-13
影响因子：
4.1
通讯作者：
Ouwens, D. Margriet
中科院分区：
文献类型：
--
作者：
Baan, Bart;van der Zon, Gerard C. M.;Ouwens, D. Margriet
生长因子通过Thr69和Thr71的顺序磷酸化激活ATF2，其中ATF2-Thr71磷酸化先于ATF2-Thr69 + 71磷酸化的诱导。在这里，我们研究了jnk1,2缺陷胚胎成纤维细胞血清诱导的两步atf2磷酸化的机制。通过阴离子交换层析，ERK1/2和p38被鉴定为atf2激酶。抑制剂研究和核定位实验表明，ERK1/2和p38的顺序核出现决定了血清对ATF2-Thr71和ATF2-Thr69 + 71磷酸化的诱导作用。2009爱思唯尔爱尔兰有限公司版权所有。
Growth factors activate ATF2 via sequential phosphorylation of Thr69 and Thr71, where the ATF2-Thr71-phosphorylation precedes the induction of ATF2-Thr69 + 71-phosphorylation. Here, we studied the mechanisms contributing to serum-induced two-step ATF2-phosphorylation in JNK 1,2-deficient embryonic fibroblasts. Using anion exchange chromatography, ERK1/2 and p38 were identified as ATF2-kinases in vitro. Inhibitor studies as well as nuclear localization experiments show that the sequential nuclear appearance of ERK1/2 and p38 determines the induction of ATF2-Thr71 and ATF2-Thr69 + 71 phosphorylation in response to serum. (C) 2009 Elsevier Ireland Ltd. All rights reserved.