Ionic composition of endolymph in teleosts: origin and importance of endolymph alkalinity.
Ionic composition of endolymph in teleosts: origin and importance of endolymph alkalinity.
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硬骨鱼内淋巴的离子组成：内淋巴碱度的起源和重要性。
DOI：
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发表时间：
1997
影响因子：
2.8
通讯作者：
Gilles Boeuf
中科院分区：
文献类型：
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作者：
Patrick Payan;Hans Kossmann;A. Watrin;N. Mayer;Gilles Boeuf
比较了鲑鱼和大比目鱼血浆和内耳内淋巴中的离子(Na+、K+、Cl-、PO4(3-)、pH)、总二氧化碳、总钙和蛋白质浓度。在两种鱼中，囊状内淋巴具有高K+和总CO2水平的特点，而在鲑鱼中具有碱性pH的特点。采用球囊封闭的滴定技术，研究了鲑鱼球囊上皮细胞质子分泌的动力学特征。质子的分泌速度强烈地依赖于林格氏溶液的pH，当pH低于7.2时，质子的分泌停止。质子分泌是由一种能量依赖的机制驱动的，该机制涉及基侧哇巴因敏感的Na+/K+交换器。乙酰唑胺可部分抑制质子分泌，无Na(+)的Ringer或1 mmol L-1阿米洛利可完全抑制质子分泌。提出了一个细胞模型，强调了通过球囊上皮进行质子交换的重要性，以解释内淋巴pH的调节，内淋巴pH是耳石钙沉积的关键因素。
Ionic (Na+, K+, Cl-, PO4(3-), pH), total CO2, total calcium and protein concentrations in the plasma and endolymph of the inner ear were compared in trout Oncorhynchus mykiss and turbot Scophthalmus maximus. In both species, saccular endolymph was characterized by high levels of K+ and total CO2 and in trout by an alkaline pH. The kinetic characteristics of proton secretion across the saccular epithelium of trout were investigation using a titration technique in which isolated saccules were mounted as closed sacs. The rate of proton secretion depends strongly on the pH of the Ringer's solution and secretion stops at a pH below 7.2. Proton secretion is driven by an energy-dependent mechanism involving basolateral ouabain-sensitive Na+/K+ exchangers. Proton secretion was partially inhibited by acetazolamide and completely inhibited in Na(+)-free Ringer or in the presence of 1 mmol l-1 amiloride. A cellular model stressing the importance of proton exchange through the saccular epithelium is proposed to explain the regulation of endolymph pH, a crucial factor for the deposition of otolith calcium.
V-ATP酶在肾上皮H运输中的作用。
DOI：
10.1242/jeb.172.1.205
发表时间：
1992
期刊：
The Journal of experimental biology
影响因子：
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作者：
Gluck,S;Nelson,R
通讯作者：
Nelson,R