A renewable potentiometric immunosensor based on Fe3O4 nanoparticles immobilized anti-IgG
A renewable potentiometric immunosensor based on Fe3O4 nanoparticles immobilized anti-IgG
复制标题
基于 Fe3O4 纳米颗粒固定化抗 IgG 的可再生电位免疫传感器
DOI：
10.1002/elan.200704094
复制
发表时间：
2008-04-01
期刊：
影响因子：
3
通讯作者：
Gao, Huding
中科院分区：
文献类型：
--
作者：
Li, Jianping;Gao, Huding
利用纳米Fe3O4颗粒磁吸附羊抗人免疫球蛋白抗体，研制了一种可再生的检测免疫球蛋白G的电位型免疫传感器。为了制备传感器敏感膜，在纳米Fe3O4颗粒表面键合了半胱氨酸。采用传统的戊二醛交联法，将半胱氨酸功能化的磁性纳米颗粒吸附在固体石蜡碳糊电极表面，实现了抗免疫球蛋白G的共价固定化。该传感器对人免疫球蛋白G的响应范围为0.1~1.2 ng/m L，检出限为0.023 ng/m L。该方法可以方便地制作基于磁性纳米颗粒的免疫传感器。它使用方便，更新容易，成本相对较低。该可再生电位型免疫传感器具有较好的稳定性和较高的灵敏度，可广泛应用于临床诊断、疾病监测和环境研究等领域。
A renewable potentiometric immunosensor for detection of immunoglobulin G (IgG) has been developed by magnetic force attraction of Fe3O4 nanoparticles immobilized goat-anti-human IgG antibody. For preparing sensitive film of the sensor, cysteine was bonded on the nano-Fe3O4 particles surface. The cysteine functionalized magnetic nanoparticles was attracted on a solid paraffin carbon paste electrode surface to covalently immobilize of anti-immunoglobulin G (anti-IgG) by employing a conventional glutaraldehyde-crosslinking method. The immunosensor showed a specific response to human immunoglobulin G in the range of 0.1-1.2 ng/mL with a detection limit of 0.023 ng/mL. The immunosensor based on the magnetic nanoparticles was made easily by this method. It can be used expediently, renewed easily and low-cost relatively. The renewable potentiometric immunosensor with better stability and higher sensitivity can be employed extensively in clinical diagnosis, monitoring of disease and environmental studies and etc.