Nonmuscle myosin IIB is involved in the guidance of fibroblast migration
Nonmuscle myosin IIB is involved in the guidance of fibroblast migration
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DOI：
10.1091/mbc.e03-06-0359
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发表时间：
2004-03-01
影响因子：
3.3
通讯作者：
Wang, YL
中科院分区：
文献类型：
--
作者：
Lo, CM;Buxton, DB;Wang, YL
虽然已知肌球蛋白II在细胞迁移中起重要作用，但对其具体功能知之甚少。我们已经解决了肌球蛋白II，肌球蛋白IIB的亚型之一的功能，通过分析成纤维细胞的运动和机械特性，这种蛋白质已被消融的基因中断。肌球蛋白IIB空细胞显示多个不稳定和混乱的突起，虽然他们仍然能够产生很大一部分的牵引力培养时，柔性聚丙烯酰胺基板。然而，相对于细胞迁移的方向，牵引力是高度紊乱的。细胞迁移模式的分析表明，速度的增加和持久性的降低，这可能是负责的缺陷，在定向运动所证明的Boyden室。此外，与对照细胞不同，突变细胞对机械信号（如压缩力和基底刚性变化）没有反应。免疫荧光染色表明肌球蛋白IIB优先沿着细胞内部区域的应力纤维定位。我们的研究结果表明，肌球蛋白IIB是参与推动，但在指导细胞运动，通过协调活动和稳定细胞极性。
Although myosin II is known to play an important role in cell migration, little is known about its specific functions. We have addressed the function of one of the isoforms of myosin II, myosin IIB, by analyzing the movement and mechanical characteristics of fibroblasts where this protein has been ablated by gene disruption. Myosin IIB null cells displayed multiple unstable and disorganized protrusions, although they were still able to generate a large fraction of traction forces when cultured on flexible polyacrylamide substrates. However, the traction forces were highly disorganized relative to the direction of cell migration. Analysis of cell migration patterns indicated an increase in speed and decrease in persistence, which were likely responsible for the defects in directional movements as demonstrated with Boyden chambers. In addition, unlike control cells, mutant cells failed to respond to mechanical signals such as compressing forces and changes in substrate rigidity. Immunofluorescence staining indicated that myosin IIB was localized preferentially along stress fibers in the interior region of the cell. Our results suggest that myosin IIB is involved not in propelling but in directing the cell movement, by coordinating protrusive activities and stabilizing the cell polarity.