Lipid Membrane Deformation Accompanied by Disk-to-Ring Shape Transition of Cholesterol-Rich Domains.
Lipid Membrane Deformation Accompanied by Disk-to-Ring Shape Transition of Cholesterol-Rich Domains.
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DOI：
10.1021/jacs.5b04559
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发表时间：
2015-07
影响因子：
15
通讯作者：
Yong-Sang Ryu;Daehan Yoo;Nathan J. Wittenberg;Luke R. Jordan;S. Lee;A. Parikh;Sang-Hyun Oh
中科院分区：
文献类型：
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作者：
Yong-Sang Ryu;Daehan Yoo;Nathan J. Wittenberg;Luke R. Jordan;S. Lee;A. Parikh;Sang-Hyun Oh
在囊泡出芽或内吞过程中，生物膜经历一系列脂质和蛋白质介导的变形，涉及富含胆固醇的脂筏。如果高弯曲刚度的脂筏变得局限于初始弯曲膜拓扑结构，如芽颈界面，则可以预期它们改革为环形筏。在这里，我们报告的观察的一个模型膜的几何约束的情况下，一个盘到环形状的形态化学转变。筏形转变是由横向组成的异质性触发，并伴随着膜变形在垂直方向上，这是由高度敏感的荧光干涉显微镜检测。我们的研究结果表明，一个平面的膜可以成为弯曲的动态变化，在当地的化学成分和形状转变的胆固醇丰富的领域。
During vesicle budding or endocytosis, biomembranes undergo a series of lipid- and protein-mediated deformations involving cholesterol-enriched lipid rafts. If lipid rafts of high bending rigidities become confined to the incipient curved membrane topology such as a bud-neck interface, they can be expected to reform as ring-shaped rafts. Here, we report on the observation of a disk-to-ring shape morpho-chemical transition of a model membrane in the absence of geometric constraints. The raft shape transition is triggered by lateral compositional heterogeneity and is accompanied by membrane deformation in the vertical direction, which is detected by height-sensitive fluorescence interference contrast microscopy. Our results suggest that a flat membrane can become curved simply by dynamic changes in local chemical composition and shape transformation of cholesterol-rich domains.