Proteome-wide Analysis of Cellular Response to Ultraviolet Light for Biomaterial Synthesis and Modification
Proteome-wide Analysis of Cellular Response to Ultraviolet Light for Biomaterial Synthesis and Modification
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DOI：
10.1021/acsbiomaterials.9b00177
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发表时间：
2019-05-01
影响因子：
5.8
通讯作者：
DeForest, Cole A.
中科院分区：
文献类型：
--
作者：
Ruskowitz, Emily R.;DeForest, Cole A.
虽然生物材料界已广泛利用近紫外（UV）光来制造和修饰用于3D细胞培养的支架，但尚未对此类光对细胞功能的下游影响进行彻底检查。在这里，我们研究了常见的光处理对NIH3T3成纤维细胞和人间充质干细胞（hMSCs）的全球影响，这些细胞类型经常用于组织工程。不变的增殖率，没有凋亡诱导，和一个不变的蛋白质组低剂量365 nm光暴露后观察，这意味着近紫外线为基础的自由基光化学可以利用在生物材料系统中，而不会有害地影响细胞的命运。
Though the biomaterials community has widely utilized near-ultraviolet (UV) light to make and modify scaffolds for 3D cell culture, thorough examination of the downstream effects of such light on cell function has not been performed. Here, we investigate the global effects of common light treatments on NIH3T3 fibroblasts and human mesenchymal stem cells (hMSCs), cell types regularly employed in tissue engineering. Unchanged proliferation rates, an absence of apoptotic induction, and an unaltered proteome following low-dose 365 nm light exposure are observed, implying that near-UV-based radical-free photochemistries can be exploited in biomaterial systems without deleteriously affecting cell fate.